Probe:	VEGF cDNA6
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS10: embryo endoderm	Present		2C
TS10: extraembryonic endoderm	Present		2C

Structure	Level	Pattern	Image	Note
TS12: Reichert's membrane	Present		3A
TS12: embryo endoderm	Present		3A
TS12: yolk sac endoderm	Present		3A
TS12: yolk sac	Present	Regionally restricted	3A	Expressed in a single squamous-like layer of mesothelial cells covering the inner part.
TS12: allantois	Present	Regionally restricted	3A	Expressed in a single squamous-like layer of mesothelial cells covering the allantois.

Structure	Level	Pattern	Image	Note
TS12: Reichert's membrane	Present		3E
TS12: embryo endoderm	Present		3E
TS12: yolk sac endoderm	Present		3E
TS12: yolk sac	Present	Regionally restricted	3E	Expressed in a single squamous-like layer of mesothelial cells covering the inner part.
TS12: allantois	Present	Regionally restricted	3E	Expressed in a single squamous-like layer of mesothelial cells covering the allantois.

Structure	Level	Pattern	Image	Note
TS13: primary trophoblast giant cell	Very strong		4C/D
TS13: cardiac muscle tissue	Strong		4C/D
TS13: gut endoderm	Strong		4C/D
TS13: amnion ectoderm	Present	Widespread	4C/D
TS13: embryo mesenchyme	Present	Widespread	4C/D

Structure	Level	Pattern	Image	Note
TS20: trophoblast giant cell	Present		5C
TS20: embryo ectoderm	Weak	Regionally restricted	5C	Expressed in neural ectoderm.
TS20: head mesenchyme	Weak		5C

Structure	Level	Pattern	Image	Note
TS28: placenta	Present	Regionally restricted	5C	Expressed in the large stroma cells.

Structure	Level	Pattern	Image	Note
TS23: adrenal gland	Strong		7A
TS23: femur	Strong		7A
TS23: head bone	Strong		7A
TS23: islets of Langerhans	Strong		7A
TS23: pituitary gland	Strong		7A
TS23: rib	Strong		7A
TS23: vertebra	Strong		7A
TS23: lymphatic system	Moderate	Regionally restricted	7A	Expressed in the lymphoid aggregates in the gastrointestinal tract.
TS23: brain ventricular layer	Present		7A
TS23: diencephalon	Present		7A
TS23: olfactory lobe	Present		7A
TS23: brain	Present	Regionally restricted	7A	Expressed in the ependymal layer.

Structure	Level	Pattern	Image	Note
TS23: renal corpuscle	Strong	Regionally restricted	8A	Expressed in the epithelium.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Single cells		Expressed in single large cells in the stroma.

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Strong			After E10.5, expression was maintained, albeit at lower levels.

Structure	Level	Pattern	Image	Note
TS22: adrenal gland	Strong
TS22: pituitary gland	Strong
TS22: pancreas	Strong	Regionally restricted		Expressed in the islets of Langerhans.
TS22: lymphatic system	Moderate	Regionally restricted		Expressed in the lymphoid aggregates in the gastrointestinal tract.
TS22: brain ventricular layer	Present
TS22: diencephalon	Present
TS22: femur cartilage condensation	Present
TS22: head bone	Present
TS22: olfactory lobe	Present
TS22: rib cartilage condensation	Present
TS22: vertebra	Present
TS22: brain	Present	Regionally restricted		Expressed in the ependymal layer.
TS22: renal corpuscle	Present	Regionally restricted		Expressed in the epithelium.

Structure	Level	Pattern	Image	Note
TS23: choroid plexus	Strong	Regionally restricted		Expressed in the epithelium.