Gene Expression Data
Assay Details
Assay
|
Reference:
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J:209070
Glaser A, et al., Tbx4 interacts with the short stature homeobox gene Shox2 in limb development. Dev Dyn. 2014 May;243(5):629-39
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Assay type:
|
RNA in situ
|
MGI Accession ID:
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MGI:5912540
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Gene symbol:
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Tbx4
|
Gene name:
|
T-box 4
|
Probe:
|
Tbx4 cDNA4
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Results
|
Specimen
2Af:
embryonic day 9.5
(more )
Specimen
2Af:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2Ag:
embryonic day 10.5
(more )
Specimen
2Ag:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud |
Present |
Regionally restricted |
2Ag
|
Expressed in a discrete localized region of the proximal limb bud.
|
TS17: hindlimb bud |
Strong |
Widespread |
2Ag
|
Expressed in the entire hindlimb bud.
|
Specimen
2Ah:
embryonic day 11.5
(more )
Specimen
2Ah:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: forelimb bud |
Present |
Widespread |
2Ah
|
Decreased expression. Expressed more diffusely in the distal part.
|
TS19: hindlimb bud |
Present |
|
2Ah
|
Expressed in the entire hindlimb bud.
|
Specimen
2Ai:
embryonic day 12.5
(more )
Specimen
2Ai:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: forelimb |
Present |
Regionally restricted |
2Ai
|
Expression proceeds distally.
|
TS20: hindlimb |
Present |
Widespread |
2Ai
|
Expressed in the entire hindlimb bud.
|
Specimen
2Aj:
embryonic day 13.5
(more )
Specimen
2Aj:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: forelimb |
Present |
Regionally restricted |
2Aj
|
Expression proceeds distally.
|
TS21: hindlimb |
Present |
Regionally restricted |
2Aj
|
Expression is more distally restricted.
|
Specimen
2Bb:
embryonic day 10.5
(more )
Specimen
2Bb:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud |
Present |
Regionally restricted |
2Bb
|
Expressed in the proximal region, overlaping with the broader of Shox2 expression domain.
|
Specimen
2Bb':
embryonic day 10.5
(more )
Specimen
2Bb':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: hindlimb bud mesenchyme |
Present |
Regionally restricted |
2Bb'
|
Expression broadly overlaps with Shox2 throughout the proximal limb mesenchyme.
|
Specimen
2Be:
embryonic day 11.5
(more )
Specimen
2Be:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: forelimb bud mesenchyme |
Present |
Regionally restricted |
2Be
|
Expressed in a region corresponding to a Col2a1-positive cartilaginous element of the dorsal forelimb. Expression patterns of Shox2 and Tbx4 are mutually exclusive.
|
Specimen
2Be':
embryonic day 11.5
(more )
Specimen
2Be':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: hindlimb bud mesenchyme |
Present |
Regionally restricted |
2Be'
|
Expression broadly overlaps with Shox2 throughout the proximal limb mesenchyme.
|
Specimen
2Bh:
embryonic day 12.5
(more )
Specimen
2Bh:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: forelimb pre-cartilage condensation |
Present |
Regionally restricted |
2Bh
|
Expressed in a region corresponding to a Col2a1-positive cartilaginous element of the dorsal forelimb. Expression patterns of Shox2 and Tbx4 are mutually exclusive.
|
Specimen
2Bh':
embryonic day 12.5
(more )
Specimen
2Bh':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hindlimb mesenchyme |
Present |
Regionally restricted |
2Bh'
|
Expressed in areas surrounding the proximal cartilaginous elements.
|
Specimen
3Aa:
embryonic day 10.5
(more )
Specimen
3Aa:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3Ab:
(close )
Genetic Background:
|
involves: 129 * C57BL/6 * CD-1
|
Mutant Allele(s):
|
Shox2tm1Acgg/Shox2tm1Acgg
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud |
Present |
|
3Ab
|
No altered expression compared with the wild-type.
|
Specimen
3Ac:
embryonic day 11.5
(more )
Specimen
3Ac:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3Ad:
(close )
Genetic Background:
|
involves: 129 * C57BL/6 * CD-1
|
Mutant Allele(s):
|
Shox2tm1Acgg/Shox2tm1Acgg
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: forelimb bud |
Present |
|
3Ad
|
Increased expression in the specific dorsal expressing region, compared with the wild-type.
|
Specimen
3Ae:
embryonic day 12.5
(more )
Specimen
3Ae:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3Af:
(close )
Genetic Background:
|
involves: 129 * C57BL/6 * CD-1
|
Mutant Allele(s):
|
Shox2tm1Acgg/Shox2tm1Acgg
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: forelimb |
Present |
|
3Af
|
Increased expression in the specific dorsal expressing region, compared with the wild-type.
|
Specimen
3Ag:
embryonic day 13.5
(more )
Specimen
3Ag:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3Ah:
(close )
Genetic Background:
|
involves: 129 * C57BL/6 * CD-1
|
Mutant Allele(s):
|
Shox2tm1Acgg/Shox2tm1Acgg
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: forelimb |
Present |
|
3Ah
|
Increased expression in the specific dorsal expressing region, compared with the wild-type.
|
Specimen
not shown:
embryonic day 10.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129 * C57BL/6 * CD-1
|
Mutant Allele(s):
|
Shox2tm1Acgg/Shox2tm1Acgg
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: hindlimb bud |
Present |
|
|
No altered expression compared with the wild-type.
|
Specimen
not shown:
embryonic day 11.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129 * C57BL/6 * CD-1
|
Mutant Allele(s):
|
Shox2tm1Acgg/Shox2tm1Acgg
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: hindlimb bud |
Present |
|
|
No altered expression compared with the wild-type.
|
Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129 * C57BL/6 * CD-1
|
Mutant Allele(s):
|
Shox2tm1Acgg/Shox2tm1Acgg
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hindlimb |
Present |
|
|
No altered expression compared with the wild-type.
|
Specimen
not shown:
embryonic day 13.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129 * C57BL/6 * CD-1
|
Mutant Allele(s):
|
Shox2tm1Acgg/Shox2tm1Acgg
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: hindlimb |
Present |
|
|
No altered expression compared with the wild-type.
|
|