Antibody:	anti-DACH1
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Level	Pattern	Image	Note
TS22: coronary artery	Present	Non-Uniform	2B right DACH1 , 2B left , 2B right merge	Expressed exclusively in endothelial cells. Expression was easily detected in the endothelial nuclei of vessels undergoing arterial remodeling, but quantification revealed levels to be lower than the nonremodeling capillary vessels (Fig. 2B').
TS22: heart	Present		2B right merge , 2B left , 2B right DACH1

Structure	Level	Pattern	Image	Note
TS23: coronary artery	Present		2C right merge , 2C right DACH1 , 2C left	Significantly decreased expression in the larger arteries that had emerged (summarized in Fig. 2C'). High expression in smaller vessels and low expression in large arteries.
TS23: heart	Present		2C left , 2C right merge , 2C right DACH1

Structure	Level	Pattern	Image	Note
TS24: coronary artery	Present	Non-Uniform	2D right DACH1 , 2D left , 2D right merge	More extensively decreased expression in arteries had completed the remodeling phase. High expression in smaller vessels and low expression in large arteries.
TS24: heart	Present		2D right DACH1 , 2D right merge , 2D left

Structure	Level	Pattern	Image	Note
TS28: coronary artery	Present	Non-Uniform	2E right , 2E left	High expression in smaller vessels and low expression in large arteries.
TS28: heart	Present		2E right , 2E left

Structure	Level	Pattern	Image	Note
TS23: coronary artery	Present		3A upper left , 3A upper right	Expressed in endothelial cells.
TS23: heart	Present		3A upper right , 3A upper left

Structure	Level	Pattern	Image	Note
TS23: coronary artery	Absent		3A lower left , 3A lower right

Structure	Level	Pattern	Image	Note
TS28: retinal artery	Present	Regionally restricted	7D lower , 7D upper	Expression was significantly higher in endothelial cells specifically at branch points.
TS28: retina	Present		7D upper , 7D lower

Structure	Level	Pattern	Image	Note
TS27: coronary artery	Present		7F upper , 7F lower	Expression was up-regulated at branch points.
TS27: heart	Present		7F lower , 7F upper

Structure	Level	Pattern	Image	Note
TS28: retina blood vessel	Present	Non-Uniform	S2_middle , S2_right upper , S2_right lower	High expression in smaller vessels and low expression in large arteries.
TS28: retinal artery	Weak		S2_right lower , S2_middle
TS28: retinal vein	Strong		S2_middle , S2_right upper

Structure	Level	Pattern	Image	Note
TS28: retinal artery	Present		S6B upper left , S6B upper right	Expression was significantly higher in endothelial cells specifically at branch points.
TS28: retina	Present		S6B upper left , S6B upper right

Structure	Level	Pattern	Image	Note
TS28: retinal artery	Present		S6B lower right , S6B lower left	Expression was significantly higher in endothelial cells specifically at branch points.
TS28: retina	Present		S6B lower right , S6B lower left