Gene Expression Data
Assay Details
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Assay
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Reference:
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J:33225
Wu JE, et al., Differential expression of integrin alpha subunits supports distinct roles during lung branching morphogenesis. Dev Dyn. 1996 Jun;206(2):169-81
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6147349
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Gene symbol:
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Itga2
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Gene name:
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integrin alpha 2
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Antibody:
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Ha1/29
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to Cy3
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Results
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Specimen
4B:
embryonic day 14.5
(more  )
Specimen
4B:
(close  )
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Genetic Background:
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involves: BALB/c * C3H
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lung epithelium |
Present |
Non-Uniform |
4B
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Expression was high at branch tips and markedly decreased to an undetectable level distal to the tips.
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| TS22: lung mesenchyme |
Present |
Regionally restricted |
4B
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Expression was detected in mesenchymal cells that express Col4a1/Col4a2. The immunopositive cells appeared to align into primitive tubules.
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Specimen
4E:
embryonic day 14.5
(more )
Specimen
4E:
(close )
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Genetic Background:
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involves: BALB/c * C3H
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lung epithelium |
Present |
Non-Uniform |
4E
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Expression was high at branch tips and markedly decreased to an undetectable level distal to the tips. Some of the immunopositive epithelial cells appeared disorganized and displayed irregular cell shapes. In some areas, immunopositive processes from these cells interdigitated with neighboring immunopositive mesenchymal cells.
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| TS22: lung mesenchyme |
Present |
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4E
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Expression co-localized with laminin in the mesenchyme.
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Specimen
not shown:
embryonic day 10.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: BALB/c * C3H
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: lung epithelium |
Present |
Non-Uniform |
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Expression was high at branch tips and markedly decreased to an undetectable level distal to the tips.
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Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: BALB/c * C3H
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: lung epithelium |
Present |
Non-Uniform |
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Expression was high at branch tips and markedly decreased to an undetectable level distal to the tips. Beginning at E12.5, some of the immunopositive epithelial cells appeared disorganized and displayed irregular cell shapes. In some areas, immunopositive processes from these cells interdigitated with neighboring immunopositive mesenchymal cells.
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| TS20: lung mesenchyme |
Present |
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Specimen
not shown:
embryonic day 16.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: BALB/c * C3H
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: lung epithelium |
Present |
Non-Uniform |
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Expression was high at branch tips and markedly decreased to an undetectable level distal to the tips. Some of the immunopositive epithelial cells appeared disorganized and displayed irregular cell shapes. In some areas, immunopositive processes from these cells interdigitated with neighboring immunopositive mesenchymal cells.
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| TS24: lung mesenchyme |
Present |
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Analysis Tools