Gene Expression Data
Assay Details
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Assay
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Reference:
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J:119175
Donner AL, et al., Sox2 and Pou2f1 interact to control lens and olfactory placode development. Dev Biol. 2007 Mar 15;303(2):784-799
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6159122
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Gene symbol:
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Sox2
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Gene name:
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SRY (sex determining region Y)-box 2
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Antibody:
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anti-Sox2
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Detection system:
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Alexa Fluor 488
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Results
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Specimen
2D:
embryonic day 8.5
(more  )
Note: 12 somites
Specimen
2D:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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12 somites
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Specimen
2E:
embryonic day 9.0
(more )
Note: 15 somites
Specimen
2E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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15 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: lens pre-placode |
Present |
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2E
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Expression detected in presumptive lens ectoderm at 15 somite stage.
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Specimen
2F:
embryonic day 9.0
(more )
Note: 17 somites
Specimen
2F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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17 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: lens pre-placode |
Present |
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2F
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Expression maintained in presumptive lens ectoderm through subsequent preplacodal stages.
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Specimen
3B,C:
embryonic day 9.5
(more )
Note: Double labeled: green - Sox2; red - Pax6 (assay MGI:6159121).
Specimen
3B,C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Sox2; red - Pax6 (assay MGI:6159121).
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Specimen
3E,F:
embryonic day 11.5
(more )
Note: Double labeled: green - Sox2; red - Pax6 (assay MGI:6159121).
Specimen
3E,F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Sox2; red - Pax6 (assay MGI:6159121).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: lens vesicle |
Present |
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3F
,
3E
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Expression throughout placode invagination and lens vesicle formation.
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Specimen
not shown:
embryonic day 13.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lens |
Weak |
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Upon differentiation, expression down-regulated throughout the lens.
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Specimen
4B:
embryonic day 9.0
(more )
Specimen
4B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4E:
embryonic day 10.5
(more )
Specimen
4E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: nasal pit |
Present |
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4E
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Expression in most cells of nasal pit.
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Specimen
4H:
embryonic day 12.5
(more )
Specimen
4H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: nasal epithelium |
Present |
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4H
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Expression in early derivatives of nasal placode, including nasal epithelium.
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Specimen
4K:
embryonic day 12.5
(more )
Specimen
4K:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: vomeronasal organ |
Present |
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4K
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Expression in early derivatives of nasal placode, including developing vomeronasal organ.
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Specimen
6C:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Pou2f1tm1Shrp/Pou2f1tm1Shrp
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: lens pit |
Present |
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6C
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Authors report normal pattern of expression.
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Specimen
6F:
embryonic day 10.5; Pax6Sey-Neu/Pax6 +
(more )
Specimen
6F:
(close )
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Genetic Background:
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involves: 102 * C3H * C3H/HeN
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Mutant Allele(s):
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Pax6Sey-Neu/Pax6+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6I:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C3H/HeN * C57BL/6
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Mutant Allele(s):
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Pou2f1tm1Shrp/Pou2f1tm1Shrp
Sox2tm1.1Vep/Sox2+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: lens pit |
Present |
Regionally restricted |
6I
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In mildly affected eyes, authors report a small domain of expression separate from the surface ectoderm, indicating presence of a small lens primordium.
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Specimen
6L:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C3H/HeN * C57BL/6
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Mutant Allele(s):
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Pou2f1tm1Shrp/Pou2f1tm1Shrp
Sox2tm1.1Vep/Sox2+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6O:
(close )
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Genetic Background:
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involves: 102 * C3H * C3H/HeN
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Mutant Allele(s):
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Pax6Sey-Neu/Pax6Sey-Neu
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: optic cup |
Present |
Regionally restricted |
6O
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Authors report expression absent from distal optic vesicle.
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Specimen
7G:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Pou2f1tm1Shrp/Pou2f1tm1Shrp
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: olfactory placode |
Present |
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7G
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Authors state nasal placodes were induced and expression was normal.
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Specimen
7J:
(close )
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Genetic Background:
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involves: 102 * C3H * C3H/HeN
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Mutant Allele(s):
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Pax6Sey-Neu/Pax6Sey-Neu
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Sox2; red - E-cadherin (Cdh1 assay MGI:6159125).
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Specimen
7K:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C3H/HeN * C57BL/6
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Mutant Allele(s):
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Pou2f1tm1Shrp/Pou2f1tm1Shrp
Sox2tm1.1Vep/Sox2+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Sox2; red - E-cadherin (Cdh1 assay MGI:6159125).
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Analysis Tools