Probe:	AP-2alpha cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS21: midbrain	Present		5C	Expression is in a distict region of the anterior midbrain and is weaker in the dorsal and anteriolateral areas.
TS21: dorsal root ganglion	Present		5C
TS21: hair follicle	Present		5C
TS21: inferior vagus ganglion	Present		5C
TS21: medulla oblongata	Present		5C
TS21: olfactory epithelium	Present		5C
TS21: oral region	Present		5C
TS21: paravertebral ganglion	Present		5C
TS21: pons	Present		5C
TS21: skin	Present		5C
TS21: spinal cord	Present		5C
TS21: midbrain roof plate	Present	Regionally restricted	5C	Expression is in a distinct layer of cells located within the roof of the midbrain.
TS21: hindbrain	Present	Regionally restricted	5C	Expression is in the area around the fourth ventricle.
TS21: cerebellum	Present	Regionally restricted	5C	Expression includes the midbrain/cerebellar junction.
TS21: spinal cord mantle layer	Present	Regionally restricted	5C	Expression is in the dorsolateral mantle layer.

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	5E	Expression is in a distict region of the anterior midbrain and is weaker in the dorsal and anteriolateral areas. Expression is absent in the layer of undifferentiated mitotic cells coating the innermost surface.
TS23: hindbrain	Present	Regionally restricted	5E	Expression is absent in the layer of undifferentiated mitotic cells coating the innermost surface.

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	5G	Expression is in a distict region of the anterior midbrain and is weaker in the dorsal and anteriolateral areas. Expression is absent in the layer of undifferentiated mitotic cells coating the innermost surface.
TS23: hindbrain	Present	Regionally restricted	5G	Expression is absent in the layer of undifferentiated mitotic cells coating the innermost surface.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Moderate
TS21: metanephros	Moderate
TS21: skin	Moderate
TS21: spinal cord	Moderate
TS21: dorsal root ganglion	Weak
TS21: eye	Weak
TS21: hindbrain	Weak
TS21: midbrain	Weak
TS21: olfactory epithelium	Weak
TS21: forebrain	Absent
TS21: heart	Absent
TS21: liver	Absent
TS21: tongue	Absent

Structure	Level	Pattern	Image	Note
TS23: cerebellum	Moderate
TS23: metanephros	Moderate
TS23: skin	Moderate
TS23: spinal cord	Moderate
TS23: dorsal root ganglion	Weak
TS23: eye	Weak
TS23: hindbrain	Weak
TS23: midbrain	Weak
TS23: olfactory epithelium	Weak
TS23: nose	Weak	Scattered		Expression is scattered around the nasal cavity.
TS23: forebrain	Absent
TS23: heart	Absent
TS23: liver	Absent
TS23: spleen primordium	Absent
TS23: sympathetic ganglion	Absent
TS23: tongue	Absent