Antibody:	Anti-Tubb3
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Structure	Level	Pattern	Image	Note
TS20: small intestine	Present	Regionally restricted	2M	Expression was seen in neurites.

Structure	Level	Pattern	Image	Note
TS20: small intestine	Present	Regionally restricted	2N	Expression was detected in neurites similar to in wild type.

Structure	Level	Pattern	Image	Note
TS20: large intestine	Present	Regionally restricted	2O	Expression in neurites in the mid-colon Sox10 immunopositive neural crest cell wavefront was variable. In this sample, very few Tubb3 immunopositive neurites were detected.

Structure	Level	Pattern	Image	Note
TS20: large intestine	Present	Regionally restricted	2P	Expression in neurites in the mid-colon Sox10 immunopositive neural crest cell wavefront was variable. In this sample, most neurites at the wavefront were Tubb3 immunopositive.

Structure	Level	Pattern	Image	Note
TS20: stomach	Present	Regionally restricted	4A , 4A'	Expression was detected in the vagus nerve at the gastroesophageal junction and as vagal nerve fibers extended into the stomach and then branched into smaller bundles.
TS20: vagus nerve	Present	Regionally restricted	4A , 4A'	Expression was detected in the vagus nerve at the gastroesophageal junction and as vagal nerve fibers extended into the stomach and then branched into smaller bundles.

Structure	Level	Pattern	Image	Note
TS20: stomach	Present	Regionally restricted	4B , 4B'	Expression was detected in the vagus nerve at the gastroesophageal junction and as vagal nerve fibers extended into the stomach and then branched into smaller bundles. Phenotypic abnormalities were noted.
TS20: vagus nerve	Present	Regionally restricted	4B , 4B'	Expression was detected in the vagus nerve at the gastroesophageal junction and as vagal nerve fibers extended into the stomach and then branched into smaller bundles. Phenotypic abnormalities were noted.

Structure	Level	Pattern	Image	Note
TS20: stomach	Present	Regionally restricted	4E' , 4E	Expression was detected in the vagus nerve at the gastroesophageal junction. Vagal nerve fibers were detected only in the proximal stomach. Phenotypic abnormalities in vagal branches were noted.
TS20: vagus nerve	Present	Regionally restricted	4E' , 4E	Expression was detected in the vagus nerve at the gastroesophageal junction. Vagal nerve fibers were detected only in the proximal stomach. Phenotypic abnormalities in vagal branches were noted.

Structure	Level	Pattern	Image	Note
TS20: vagus nerve	Present	Regionally restricted	4F , 4F'	Expression was detected in the vagus nerve at the gastroesophageal junction.
TS20: stomach	Present	Regionally restricted	4F , 4F'	In 2/4 double mutant mice, vagal nerve fibers were absent from both sides of the stomach, and the only immunostaining was associated with a very small number of intrinsic neurons. In the other 2/4 double mutant mice, a small number of short vagal fibers was present on one side of the stomach only.

Structure	Level	Pattern	Image	Note
TS27: stomach	Present	Regionally restricted	4G	Expression was detected in the vagus nerve at the gastroesophageal junction and as vagal nerve fibers extended into the stomach and then branched into smaller bundles.
TS27: vagus nerve	Present	Regionally restricted	4G	Expression was detected in the vagus nerve at the gastroesophageal junction and as vagal nerve fibers extended into the stomach and then branched into smaller bundles.

Structure	Level	Pattern	Image	Note
TS27: stomach	Present	Regionally restricted	4H	Expression was detected in the vagus nerve at the gastroesophageal junction and as vagal nerve fibers extended into the stomach and then branched into smaller bundles. Phenotypic abnormalities were noted.
TS27: vagus nerve	Present	Regionally restricted	4H	Expression was detected in the vagus nerve at the gastroesophageal junction and as vagal nerve fibers extended into the stomach and then branched into smaller bundles. Phenotypic abnormalities were noted.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Present	Regionally restricted	4I	Immunopositive vagal fibers are present in the pancreas.
TS20: vagus nerve	Present	Regionally restricted	4I	Immunopositive vagal fibers are present in the pancreas.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Present	Regionally restricted	4J	Small numbers of immunopositive vagal fibers are present in the pancreas.
TS20: vagus nerve	Present	Regionally restricted	4J	Small numbers of immunopositive vagal fibers are present in the pancreas.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Absent		4K	No vagal nerve fibers were observed in the pancreas.

Structure	Level	Pattern	Image	Note
TS20: vagus nerve	Present		5A
TS20: lung	Present	Regionally restricted	5A	Immunopositive vagal fibers were detected in the lung.

Structure	Level	Pattern	Image	Note
TS20: vagus nerve	Present		5B
TS20: lung	Present	Regionally restricted	5B	Immunopositive vagal fibers were detected in the lung, but the number of fibers was reduced in the mutant.

Structure	Level	Pattern	Image	Note
TS24: diaphragm	Present	Regionally restricted	5C	Immunopositive nerve terminals were present in the costal muscle of the diaphragm.

Structure	Level	Pattern	Image	Note
TS24: diaphragm	Present	Regionally restricted	5D	Immunopositive nerve terminals were present in the costal muscle of the diaphragm. There was no noticeable difference in the mutant compared to wild type.

Structure	Level	Pattern	Image	Note
TS27: olfactory system	Present	Regionally restricted	6M , 6O	Immunopositive axons were observed projecting from the olfactory epithelium towards the olfactory bulbs.

Structure	Level	Pattern	Image	Note
TS27: olfactory system	Present	Regionally restricted	6P , 6N	Immunopositive axons were observed projecting from the olfactory epithelium towards the olfactory bulbs. There was no obvious difference in the appearance of staining in the mutant.

Structure	Level	Pattern	Image	Note
TS17: gut	Present	Regionally restricted	S3A left , S3A top right	Expression was detected in neurons. There was overlap of immunopositive cells that hybridized to the Kif1bp RNA probe.

Structure	Level	Pattern	Image	Note
TS19: small intestine	Present	Regionally restricted	S3B left , S3B top right	Expression was detected in neurons. There was overlap of immunopositive cells that hybridized to the Kif1bp RNA probe.

Structure	Level	Pattern	Image	Note
TS22: small intestine	Present	Regionally restricted	S3D top right , S3D left	Expression was detected in neurons. There was overlap of immunopositive cells that hybridized to the Kif1bp RNA probe.

Structure	Level	Pattern	Image	Note
TS22: large intestine	Present	Regionally restricted	S3E top right , S3E left	Expression was detected in neurons in the colon. There was overlap of immunopositive cells that hybridized to the Kif1bp RNA probe.