Gene Expression Data
Assay Details
Assay
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Reference:
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J:121343
Lahteenmaki M, et al., Increased apoptosis of parasympathetic but not enteric neurons in mice lacking GFRalpha2. Dev Biol. 2007 May 1;305(1):325-32
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6161697
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Gene symbol:
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Casp3
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Gene name:
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caspase 3
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Results
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Specimen
5a:
embryonic day 17.5
(more )
Note: Triple labeled: red - activated caspase-3 (Casp3); green - PGP9.5 (Uchl1 assay MGI:6161683); blue - Hoechst 33258.
Specimen
5a:
(close )
Genetic Background:
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involves: C57BL/6JOlaHsd
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: red - activated caspase-3 (Casp3); green - PGP9.5 (Uchl1 assay MGI:6161683); blue - Hoechst 33258.
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Specimen
5b:
embryonic day 17.5
(more )
Note: Triple labeled: red - activated caspase-3 (Casp3); green - PGP9.5 (Uchl1 assay MGI:6161683); blue - Hoechst 33258.
Specimen
5b:
(close )
Genetic Background:
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involves: C57BL/6JOlaHsd
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: red - activated caspase-3 (Casp3); green - PGP9.5 (Uchl1 assay MGI:6161683); blue - Hoechst 33258.
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Structure |
Level |
Pattern |
Image |
Note |
TS25: paravertebral ganglion |
Present |
Regionally restricted |
5b
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Expression in neurons of paravertebral sympathetic ganglia.
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Specimen
5d/f:
embryonic day 17.5
(more )
Note: Triple labeled: red - activated caspase-3 (Casp3); green - PGP9.5 (Uchl1 assay MGI:6161683); blue - Hoechst 33258.
Specimen
5d/f:
(close )
Genetic Background:
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involves: C57BL/6JOlaHsd
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: red - activated caspase-3 (Casp3); green - PGP9.5 (Uchl1 assay MGI:6161683); blue - Hoechst 33258.
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Structure |
Level |
Pattern |
Image |
Note |
TS25: pancreas |
Present |
Regionally restricted |
5f
,
5d
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Expression in neurons with pyknotic nuclei in intrapancreatic ganglia.
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TS25: parasympathetic ganglion |
Present |
Regionally restricted |
5f
,
5d
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Expression in neurons with pyknotic nuclei in intrapancreatic ganglia.
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Specimen
5h/j:
embryonic day 17.5; Gfra2tm1Msa/ Gfra2tm1Msa
(more )
Note: Triple labeled: red - activated caspase-3 (Casp3); green - PGP9.5 (Uchl1 assay MGI:6161683); blue - Hoechst 33258.
Specimen
5h/j:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Gfra2tm1Msa/Gfra2tm1Msa
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
|
Note:
|
Triple labeled: red - activated caspase-3 (Casp3); green - PGP9.5 (Uchl1 assay MGI:6161683); blue - Hoechst 33258.
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: pancreas |
Present |
Regionally restricted |
5h
,
5j
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Expression in neurons with pyknotic nuclei in intrapancreatic ganglia.
|
TS25: parasympathetic ganglion |
Present |
Regionally restricted |
5h
,
5j
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Expression in neurons with pyknotic nuclei in intrapancreatic ganglia.
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Specimen
5k/m:
postnatal day 0; Gfra2tm1Msa/ Gfra2tm1Msa
(more )
Note: Triple labeled: red - activated caspase-3 (Casp3); green - PGP9.5 (Uchl1 assay MGI:6161683); blue - Hoechst 33258.
Specimen
5k/m:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Gfra2tm1Msa/Gfra2tm1Msa
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: red - activated caspase-3 (Casp3); green - PGP9.5 (Uchl1 assay MGI:6161683); blue - Hoechst 33258.
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Specimen
Supplementary Figure b:
embryonic day 15.5
(more )
Note: Double labeled: red - activated caspase-3 (Casp3); green - PGP9.5 (Uchl1 assay MGI:6161683).
Specimen
Supplementary Figure b:
(close )
Genetic Background:
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involves: C57BL/6JOlaHsd
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - activated caspase-3 (Casp3); green - PGP9.5 (Uchl1 assay MGI:6161683).
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