Gene Expression Data
Assay Details
Assay
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Reference:
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J:257654
Memic F, et al., Transcription and Signaling Regulators in Developing Neuronal Subtypes of Mouse and Human Enteric Nervous System. Gastroenterology. 2018 Feb;154(3):624-636
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6164711
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Gene symbol:
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Tlx3
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Gene name:
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T cell leukemia, homeobox 3
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Antibody:
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anti-Tlx3 (rabbit)
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Detection system:
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Secondary antibody
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Assay notes:
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The authors used two antibodies to Tlx3 but did not distinguish which data was derived from which; it is possible MGI:6159161 was used to obtain some or all of these data.
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Results
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Specimen
Table 3A:
embryonic day 18.5
(more )
Specimen
Table 3A:
(close )
Genetic Background:
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either: C57BL/6 or MF1
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS26: enteric nervous system |
Present |
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Co-expression analysis was done with neurotransmitters and neuropeptides: TH, NPY and ChAT (in the stomach) and CGRP, Calb1, and Nos1 (in the small intestine). Co-expression was seen with Th and CGRP and partial co-expression with Calb1, Nos1, Npy and CHaT.
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Specimen
S3 - stomach - E11-12:
embryonic day 11.5,12.5
(more )
Note: Triple labeled: red - Tlx3; green - Elavl3/4 (to label neurons); blue - Sox10 or Sox 2 (to label enteric progenitors).
Specimen
S3 - stomach - E11-12:
(close )
Genetic Background:
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either: C57BL/6 or MF1
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Age:
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embryonic day 11.5,12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Tlx3; green - Elavl3/4 (to label neurons); blue - Sox10 or Sox 2 (to label enteric progenitors).
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Structure |
Level |
Pattern |
Image |
Note |
TS20: enteric nervous system |
Present |
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S3_TLX3 - stomach - E11-12
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Expression was seen in many neurons and a few progenitors.
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Specimen
S3 - stomach - E15-16:
embryonic day 15.5,16.5
(more )
Note: Triple labeled: red - Tlx3; green - Elavl3/4 (to label neurons); blue - Sox10 or Sox 2 (to label enteric progenitors).
Specimen
S3 - stomach - E15-16:
(close )
Genetic Background:
|
either: C57BL/6 or MF1
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Age:
|
embryonic day 15.5,16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Tlx3; green - Elavl3/4 (to label neurons); blue - Sox10 or Sox 2 (to label enteric progenitors).
|
Specimen
S3 - stomach - E18-19:
embryonic day 18.5,19.5
(more )
Note: Triple labeled: red - Tlx3; green - Elavl3/4 (to label neurons); blue - Sox10 or Sox 2 (to label enteric progenitors).
Specimen
S3 - stomach - E18-19:
(close )
Genetic Background:
|
either: C57BL/6 or MF1
|
Age:
|
embryonic day 18.5,19.5
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Sex:
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Not Specified
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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Triple labeled: red - Tlx3; green - Elavl3/4 (to label neurons); blue - Sox10 or Sox 2 (to label enteric progenitors).
|
Specimen
S3 - intestine - E11-12:
embryonic day 11.5,12.5
(more )
Note: Triple labeled: red - Tlx3; green - Elavl3/4 (to label neurons); blue - Sox10 or Sox 2 (to label enteric progenitors).
Specimen
S3 - intestine - E11-12:
(close )
Genetic Background:
|
either: C57BL/6 or MF1
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Age:
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embryonic day 11.5,12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Note:
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Triple labeled: red - Tlx3; green - Elavl3/4 (to label neurons); blue - Sox10 or Sox 2 (to label enteric progenitors).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: enteric nervous system |
Present |
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S3_TLX3 - intestine - E11-12
|
Expression was seen in many neurons and a few progenitors.
|
Specimen
S3 - intestine - E15-16:
embryonic day 15.5,16.5
(more )
Note: Triple labeled: red - Tlx3; green - Elavl3/4 (to label neurons); blue - Sox10 or Sox 2 (to label enteric progenitors).
Specimen
S3 - intestine - E15-16:
(close )
Genetic Background:
|
either: C57BL/6 or MF1
|
Age:
|
embryonic day 15.5,16.5
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Triple labeled: red - Tlx3; green - Elavl3/4 (to label neurons); blue - Sox10 or Sox 2 (to label enteric progenitors).
|
Specimen
S3 - intestine - E18-19:
embryonic day 18.5,19.5
(more )
Note: Triple labeled: red - Tlx3; green - Elavl3/4 (to label neurons); blue - Sox10 or Sox 2 (to label enteric progenitors).
Specimen
S3 - intestine - E18-19:
(close )
Genetic Background:
|
either: C57BL/6 or MF1
|
Age:
|
embryonic day 18.5,19.5
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Triple labeled: red - Tlx3; green - Elavl3/4 (to label neurons); blue - Sox10 or Sox 2 (to label enteric progenitors).
|
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