Probe:	Ptx1 cDNA3
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch mandibular component mesenchyme	Strong	Regionally restricted	1A	Strong expression in proximal mesenchyme of mandible.
TS16: oral epithelium	Present	Regionally restricted	1A	Less strong expression in distal part of oral epithelium where incisors will develop.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component mesenchyme	Strong	Regionally restricted	1B	Strong expression in mandibular mesenchyme.
TS17: oral epithelium	Present	Widespread	1B	Expression increased and extended to the totality of the oral epithelium.

Structure	Level	Pattern	Image	Note
TS20: dental placode	Present	Regionally restricted	1C	Expression in dental placode epithelium.

Structure	Level	Pattern	Image	Note
TS21: tooth bud	Present	Regionally restricted	1D	Epithelial expression during bud stage.
TS21: tooth epithelium	Present	Regionally restricted	1D	Epithelial expression during bud stage.

Structure	Level	Pattern	Image	Note
TS23: cap stage tooth	Present	Regionally restricted	1E	Epitthelial expression during cap stage.
TS23: tooth epithelium	Present	Regionally restricted	1E	Expression in all cell layers of dental epithelium. No expression in enamel knot.
TS23: enamel knot	Absent		1E

Structure	Level	Pattern	Image	Note
TS25: early bell stage tooth	Present	Regionally restricted	1F	Epithelial expression during early bell stage.
TS25: enamel organ	Present	Regionally restricted	1F	Expression only in cells of inner and outer dental (or enamel) epithelium.

Structure	Level	Pattern	Image	Note
TS26: tooth epithelium	Present	Regionally restricted	1G	Restricted expression in undifferentiated, proliferating cells of inner dental epithelium at intercuspal and cervical loop areas.
TS26: cervical loop	Present		1G

Structure	Level	Pattern	Image	Note
TS27: Hertwig's epithelial root sheath	Present	Regionally restricted	1H	Expression in epithelial cells of developing root (Hertwig's epithelium).

Structure	Level	Pattern	Image	Note
TS27: enamel organ	Present	Regionally restricted	2A	Authors state that ameloblast differentiation coincided with down regulation of expression.
TS27: molar epithelium	Present	Regionally restricted	2A	Expression persisted in first molar in cells of inner enamel epithelium in intercuspal and loop areas, as well as in Hertwig's epithelium.
TS27: Hertwig's epithelial root sheath	Present		2A
TS27: cervical loop	Present		2A

Structure	Level	Pattern	Image	Note
TS27: molar epithelium	Weak	Regionally restricted	2C	Expression down regulated in crown area of first molar.
TS27: outer enamel epithelium	Absent
TS27: stellate reticulum	Absent
TS27: stratum intermedium	Absent

Structure	Level	Pattern	Image	Note
TS27: molar epithelium	Strong	Regionally restricted	2E	Strong expression in second molar restricted to inner enamel epithelium.

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch mandibular component mesenchyme	Strong	Regionally restricted	3A	Strong expression in mandibular mesenchyme where molars will develop.

Structure	Level	Pattern	Image	Note
TS22: molar epithelium	Present	Regionally restricted	4A	Expression in epithelium of E14 molar. No expression in enamel knot.
TS22: enamel knot	Absent		4A