Probe:	Gata6 probe4
Probe preparation:	labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: duodenum epithelium	Present		1A
TS20: umbilical vein	Present		1A
TS20: Meckel's cartilage	Present	Regionally restricted	1A	Expression in walls of Meckel's cartilage
TS20: chondrocranium bone	Present	Regionally restricted	1A	Expression throughout basio-occipital bone.
TS20: cervical vertebral cartilage condensation	Present	Widespread	1A	Expression in prevertebra of cervical, thoracic, lumbar, sacral and caudal regions.
TS20: lumbar vertebral cartilage condensation	Present	Widespread	1A	Expression in prevertebra of cervical, thoracic, lumbar, sacral and caudal regions.
TS20: sacral vertebral cartilage condensation	Present	Widespread	1A	Expression in prevertebra of cervical, thoracic, lumbar, sacral and caudal regions.
TS20: sacral vertebral pre-cartilage condensation	Present	Widespread	1A	Expression in prevertebra of cervical, thoracic, lumbar, sacral and caudal regions.
TS20: tail vertebral pre-cartilage condensation	Present	Widespread	1A	Expression in prevertebra of cervical, thoracic, lumbar, sacral and caudal regions.
TS20: thoracic vertebral cartilage condensation	Present	Widespread	1A	Expression in prevertebra of cervical, thoracic, lumbar, sacral and caudal regions.

Structure	Level	Pattern	Image	Note
TS20: endocardial cushion	Strong		1B
TS20: cardiac muscle tissue	Present	Single cells	1B	Expression in isolated, individual cells within the myocardium.

Structure	Level	Pattern	Image	Note
TS20: thoracic vertebral cartilage condensation	Present	Regionally restricted	1C	Expression in prevertebra of thoracic vertebral column. In more rostral prevertebra, weaker expression in more mature centres or prevertebra which will give rise to transverse processes. No expression in center of neural arch condensations.
TS20: dorsal root ganglion	Absent		1C

Structure	Level	Pattern	Image	Note
TS20: sacral vertebral cartilage condensation	Present	Regionally restricted	1D	Expression in sacral/caudal vertebral column. Uniform expression throughout centra of prevertebra.
TS20: tail vertebral pre-cartilage condensation	Present	Regionally restricted	1D	Expression in sacral/caudal vertebral column. Uniform expression throughout centra of prevertebra.
TS20: spinal cord	Absent		1D

Structure	Level	Pattern	Image	Note
TS20: thoracic vertebral cartilage condensation	Present	Regionally restricted	1E	Expression domain in thoracic vertebral column broadly overlaps with Uncx. Uniform expression in centra of prevertebra. Expression extends into pedicle.
TS20: rib pre-cartilage condensation	Present	Regionally restricted	1E	Expression extends into head of rib.

Structure	Level	Pattern	Image	Note
TS20: cervical vertebral cartilage condensation	Present	Regionally restricted	1G	Authors report expression in superior vertebral column.
TS20: chondrocranium bone	Present	Regionally restricted	1G	Expression in dorsal region of basio-occipital bone.
TS20: C1 vertebral cartilage condensation	Present	Regionally restricted	1G	Expression in (C1) atlas and (C2) axis prevertebra. Expression in sclerotomal cells from fifth somite contributing to both prevertebra.
TS20: C2 vertebral cartilage condensation	Present	Regionally restricted	1G	Expression in (C1) atlas and (C2) axis prevertebra. Expression in sclerotomal cells from fifth somite contributing to both prevertebra.

Structure	Level	Pattern	Image	Note
TS23: femur	Present	Regionally restricted	1I	Expression largely associated with proliferating chondrocyte precursors flanking central region of hypertrophic chondrocytes. No expresssion in central region of hypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS20: radius-ulna pre-cartilage condensation	Present	Regionally restricted	5D	Expression in cartilage primordia of the radius.
TS20: humerus pre-cartilage condensation	Present	Regionally restricted	5D	Expression extends into head of rib.