Gene Expression Data
Assay Details
Assay
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Reference:
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J:137275
Yu Z, et al., Grainyhead-like factor Get1/Grhl3 regulates formation of the epidermal leading edge during eyelid closure. Dev Biol. 2008 Jul 1;319(1):56-67
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6194317
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Gene symbol:
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Grhl3
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Gene name:
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grainyhead like transcription factor 3
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Antibody:
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Anti-Get1
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
2A:
embryonic day 13.5
(more )
Specimen
2A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS21: eyelid epithelium |
Present |
Uniform |
2A
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Uniform expression in epidermal keratinocytes covering eyelid.
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Specimen
2B:
embryonic day 14.5
(more )
Note: Double labeled: green - Get-1 (Grhl3); red - DAPI staining.
Specimen
2B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Get-1 (Grhl3); red - DAPI staining.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: eyelid epithelium |
Strong |
Regionally restricted |
2B
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Expression upregulated in cluster of rounded keratinocytes initiating formation of leading edge.
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Specimen
2C:
embryonic day 14.5
(more )
Note: Double labeled: green - Get-1 (Grhl3); red - DAPI staining.
Specimen
2C:
(close )
Genetic Background:
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Not Specified
|
Age:
|
embryonic day 14.5
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Sex:
|
Not Specified
|
Type:
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section
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Fixation:
|
10% Formalin
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Get-1 (Grhl3); red - DAPI staining.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: eyelid epithelium |
Strong |
Regionally restricted |
2C
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Expression upregulated in cluster of rounded keratinocytes initiating formation of leading edge.
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Specimen
2E:
embryonic day 15.5
(more )
Note: Double labeled: green - Get-1 (Grhl3); red - DAPI staining.
Specimen
2E:
(close )
Genetic Background:
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Not Specified
|
Age:
|
embryonic day 15.5
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Sex:
|
Not Specified
|
Type:
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section
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Fixation:
|
10% Formalin
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Get-1 (Grhl3); red - DAPI staining.
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: eyelid epithelium |
Strong |
Regionally restricted |
2E
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High expression in keratinocytes of outer layer of migrating edge.
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Specimen
2F:
embryonic day 15.5
(more )
Note: Double labeled: green - Get-1 (Grhl3); red - DAPI staining.
Specimen
2F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
10% Formalin
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Get-1 (Grhl3); red - DAPI staining.
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: eyelid epithelium |
Strong |
Regionally restricted |
2F
|
High expression in keratinocytes of outer layer of migrating edge.
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Specimen
2D:
embryonic day 17.0
(more )
Note: Double labeled: green - Get-1 (Grhl3); red - DAPI staining.
Specimen
2D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 17.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
|
Paraffin
|
Note:
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Double labeled: green - Get-1 (Grhl3); red - DAPI staining.
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Structure |
Level |
Pattern |
Image |
Note |
TS25: eyelid epithelium |
Weak |
Regionally restricted |
2D
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Expression downregulated along fusion line but persists in suprabasal epidermal keratinocytes covering eyelids.
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