Gene Expression Data
Assay Details
Assay
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Reference:
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J:237110
Lee YG, et al., Coordinated lymphangiogenesis is critical in lymph node development and maturation. Dev Dyn. 2016 Dec;245(12):1189-1197
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6197052
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Gene symbol:
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Cd4
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Gene name:
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CD4 antigen
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Antibody:
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Anti-Cd4
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
2I +/+:
embryonic day 14.5
(more )
Note: Double labeled: red - Cd4; white - Pecam1 (assay MGI:6197053).
Specimen
2I +/+:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Cd4; white - Pecam1 (assay MGI:6197053).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: hemolymphoid system |
Present |
Regionally restricted |
2I Vegfc+/+
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Expression was detected in the LTi - a cluster of hematopoietic lymphoid inducer cells.
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Specimen
2I +/LacZ:
embryonic day 14.5; Vegfctm1Ali/Vegfc +
(more )
Note: Double labeled: red - Cd4; white - Pecam1 (assay MGI:6197053).
Specimen
2I +/LacZ:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Vegfctm1Ali/Vegfc+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Cd4; white - Pecam1 (assay MGI:6197053).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: hemolymphoid system |
Present |
Regionally restricted |
2I Vegfc+/LacZ
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Expression was detected in the LTi - a cluster of hematopoietic lymphoid inducer cells, which was smaller in the mutant.
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Specimen
3B P1:
postnatal day 1
(more )
Note: Triple labeled: red - Cd4; green - Lyve1 (assay MGI:6197056); blue - Pecam1 (assay MGI:6197053).
Specimen
3B P1:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Cd4; green - Lyve1 (assay MGI:6197056); blue - Pecam1 (assay MGI:6197053).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: lymph node |
Present |
Regionally restricted |
3B P1
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Expression was detected in the T-cells population, which was separate from the Lyve1+ population of macrophages and lymphatic vessels.
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Specimen
3B P2:
postnatal day 2
(more )
Note: Triple labeled: red - Cd4; green - Lyve1 (assay MGI:6197056); blue - Pecam1 (assay MGI:6197053).
Specimen
3B P2:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Cd4; green - Lyve1 (assay MGI:6197056); blue - Pecam1 (assay MGI:6197053).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: lymph node |
Present |
Regionally restricted |
3B P2
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Expression was detected in the T-cells population, which was separate from the Lyve1+ population of macrophages and lymphatic vessels.
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