Gene Expression Data
Assay Details
Assay
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Reference:
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J:264256
Bernardo AS, et al., Mammalian embryo comparison identifies novel pluripotency genes associated with the naive or primed state. Biol Open. 2018 Aug 17;7(8):bio033282
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:6202676
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Gene symbol:
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Sema6a
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Gene name:
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sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
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Probe:
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Sema6a-pA, Sema6a-pB
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Probe preparation:
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labelled with SYBR green
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Assay notes:
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Quantitative RT-PCR. Expression was normalized against Gapdh.
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Results
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E3.5
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TS4: inner cell mass
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Not Specified
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0.3 µg; total RNA |
(C57BL/6J x DBA/2J)F1 |
| Not Specified |
ICM - Inner cell mass
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E3.5
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TS4: blastocyst-stage conceptus
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Not Specified
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0.3 µg; total RNA |
(C57BL/6J x DBA/2J)F1 |
| Not Specified |
Blast - Blastocyst
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E6.25
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TS8: epiblast
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Present
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0.3 µg; total RNA |
Not Specified |
| Not Specified |
Either 129S2 or (C57BL/6J x DBA/2J)F1 mice were used. ERSE - epithelial radially symmetric epiblast.
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E7.25
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TS10: embryo
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Present
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0.3 µg; total RNA |
Not Specified |
| Not Specified |
Either 129S2 or (C57BL/6J x DBA/2J)F1 mice were used. APE - asymmetrical elongated anterior to posterior epiblast.
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Notes:
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