Gene Expression Data
Assay Details
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Assay
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Reference:
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J:263979
Harley RJ, et al., Neuronal cell adhesion molecule (NrCAM) is expressed by sensory cells in the cochlea and is necessary for proper cochlear innervation and sensory domain patterning during development. Dev Dyn. 2018 Mar 14;
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6241346
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Gene symbol:
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Nefh
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Gene name:
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neurofilament, heavy polypeptide
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Antibody:
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anti-NFH (Aves)
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Detection system:
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Secondary antibody coupled to Cy5
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Results
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Specimen
3A:
postnatal day 2
(more  )
Note: Double labeled: purple - Nefh; green - Gap43 (assay MGI:6256951).
Specimen
3A:
(close  )
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Genetic Background:
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either: CD-1 or NIH Swiss
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: purple - Nefh; green - Gap43 (assay MGI:6256951).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cochlear ganglion |
Present |
Regionally restricted |
3A
,
3C
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Expression is in the afferent fibers and is absent in efferent fibers.
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| TS27: organ of Corti |
Present |
Regionally restricted |
3A
,
3C
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Expression is in the afferent fibers and is absent in efferent fibers.
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Specimen
3D:
postnatal day 2
(more )
Note: Double labeled: purple - Nefh; green - Gap43 (assay MGI:6256951).
Specimen
3D:
(close )
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Genetic Background:
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either: CD-1 or NIH Swiss
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: purple - Nefh; green - Gap43 (assay MGI:6256951).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cochlear ganglion |
Present |
Regionally restricted |
3F
,
3D
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Expression is in the afferent fibers and is absent in efferent fibers.
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| TS27: organ of Corti |
Present |
Regionally restricted |
3F
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3D
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Expression is in the afferent fibers and is absent in efferent fibers.
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Specimen
3G:
postnatal day 2; Nrcamtm1Gmt/Nrcam +
(more )
Specimen
3G:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Nrcamtm1Gmt/Nrcam+
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3H:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Nrcamtm1Gmt/Nrcamtm1Gmt
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
7B:
postnatal day 10
(more )
Specimen
7B:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Age:
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postnatal day 10
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Present |
Regionally restricted |
7B
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Expression is in spiral ganglion neuron afferents and efferents.
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Specimen
7D:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Nrcamtm1Gmt/Nrcamtm1Gmt
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Age:
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postnatal day 10
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Present |
Regionally restricted |
7D
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Expression is in spiral ganglion neuron afferents and efferents which are disorganized in this mutant.
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Analysis Tools