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Gene Expression Data
Assay Details
Assay
Reference: J:167064 L'honore A, et al., Pitx2 defines alternate pathways acting through MyoD during limb and somitic myogenesis. Development. 2010 Nov;137(22):3847-56
Assay type: RNA in situ
MGI Accession ID: MGI:6260205
Gene symbol: Myod1
Gene name: myogenic differentiation 1
Probe: MyoD probe25
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 2C: embryonic day 11.5; Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS19: hindlimb bud Present 2C
Specimen 2D: embryonic day 11.5; Pitx2tm2Sac/Pitx2tm2Sac (more )
Structure Level Pattern Image Note
TS19: hindlimb bud Weak 2D Expression markedly decreased in Pitx2-/- embryos compared to heterozygous controls.
Specimen E10.5 not shown: embryonic day 10.5; Pitx2tm2Sac/Pitx2tm2Sac (more )
Structure Level Pattern Image Note
TS17: forelimb bud Weak Expression decreased in Pitx2-/- embryos compared to heterozygous controls.
Specimen 2E: embryonic day 11.5; Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS19: forelimb bud Present 2E
TS19: myotome Present 2E
Specimen 2F: embryonic day 11.5; Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS19: forelimb bud Weak 2F Decreased expression in E11.5 homozygous mutant forelimb compared to heterozygous controls.
TS19: myotome Present 2F Authors state myotome expression is not delayed in Pitx2-/- embryos.
Specimen S1C: embryonic day 12.5; Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS20: hindlimb Present S1C
Specimen S1D: embryonic day 12.5; Pitx2tm2Sac/Pitx2tm2Sac (more )
Structure Level Pattern Image Note
TS20: hindlimb Weak S1D Decreased expression in E11.5 homozygous mutant hindlimbs compared to heterozygous controls.
Specimen 2G: embryonic day 12.5; Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS20: forelimb Present 2G
Specimen 2H: embryonic day 12.5; Pitx2tm2Sac/Pitx2tm2Sac (more )
Structure Level Pattern Image Note
TS20: forelimb Present 2H Authors state expression is largely recovered in E12.5 forelimb buds.
Specimen E13 not shown: embryonic day 13.0; Pitx2tm2Sac/Pitx2tm2Sac (more )
Structure Level Pattern Image Note
TS21: forelimb Present Authors report expression patterns at E13.0 suggesting full recovery of expression.
Specimen 5A: embryonic day 12.5; Myf5tm2Pas/Myf5+, Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS20: forelimb Present 5A
Specimen 5B: embryonic day 12.5; Myf5tm2Pas/Myf5tm2Pas, Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS20: forelimb Present 5B Authors report expression is not affected in Myf5nLacZ/nLacZ mutant embryos.
Specimen 5C: embryonic day 12.5; Myf5tm2Pas/Myf5+, Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS20: forelimb Present 5C
Specimen 5D: embryonic day 12.5; Myf5tm2Pas/Myf5tm2Pas, Pitx2tm2Sac/Pitx2tm2Sac (more )
Structure Level Pattern Image Note
TS20: forelimb Trace 5D Authors state that inactivation of Myf5(Mrf4) in Pitx2-/- background resulted in almost complete loss of expression at E12.5.
Specimen 5E: embryonic day 12.5; Myf5tm2Pas/Myf5+, Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS20: hindlimb Present 5E
Specimen 5F: embryonic day 12.5; Myf5tm2Pas/Myf5tm2Pas, Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS20: hindlimb Present 5F Authors report expression is not affected in Myf5nLacZ/nLacZ mutant embryos.
Specimen 5G: embryonic day 12.5; Myf5tm2Pas/Myf5+, Pitx2tm2Sac/Pitx2tm2Sac (more )
Structure Level Pattern Image Note
TS20: hindlimb Present 5G
Specimen 5H: embryonic day 12.5; Myf5tm2Pas/Myf5tm2Pas, Pitx2tm2Sac/Pitx2tm2Sac (more )
Structure Level Pattern Image Note
TS20: hindlimb Trace 5H Inactivation of Myf5 in Pitx2-/- background resulted in almost complete loss of expression at E12.5.
Specimen 6A: embryonic day 12.5; Myf5tm2Pas/Myf5tm2Pas, Pitx2tm2Sac/Pitx2tm2Sac (more )
Structure Level Pattern Image Note
TS20: myotome Present 6A
Specimen 6B: embryonic day 12.5; Myf5tm2Pas/Myf5tm2Pas, Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS20: myotome Present 6B
Specimen 6C: embryonic day 12.5; Myf5tm2Pas/Myf5+, Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS20: myotome Present 6C
Specimen 6D: embryonic day 12.5; Myf5tm2Pas/Myf5tm2Pas, Pitx2tm2Sac/Pitx2tm2Sac (more )
Structure Level Pattern Image Note
TS20: myotome Trace 6D Authors report almost compete loss of expression.
Specimen S6A: embryonic day 12.5; Myf5tm2Pas/Myf5+, Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS20: myotome Present S6A
Specimen S6B: embryonic day 12.5; Myf5tm2Pas/Myf5tm2Pas, Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS20: myotome Present S6B
Specimen S6C: embryonic day 12.5; Myf5tm2Pas/Myf5+, Pitx2tm2Sac/Pitx2tm2Sac (more )
Structure Level Pattern Image Note
TS20: myotome Present S6C
Specimen S6D: embryonic day 12.5; Myf5tm2Pas/Myf5tm2Pas, Pitx2tm2Sac/Pitx2tm2Sac (more )
Structure Level Pattern Image Note
TS20: myotome Trace S6D Authors report almost compete loss of expression.
Specimen 7E: embryonic day 12.0; Pax3Sp-d/Pax3+, Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS20: myotome Present 7E
Specimen 7F: embryonic day 12.0; Pax3Sp-d/Pax3Sp-d, Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS20: myotome Present 7F
Specimen 7G: embryonic day 12.0; Pax3Sp-d/Pax3Sp-d, Pitx2tm2Sac/Pitx2tm2Sac (more )
Structure Level Pattern Image Note
TS20: myotome Present 7G Inactivation of both genes does not have a greater effect than that of either mutation.
Specimen 7H: embryonic day 12.0; Pax3Sp-d/Pax3+, Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS20: skeletal musculature Present Regionally restricted 7H Authors report expression in a muscle opposite the forelimb.
Specimen 7I: embryonic day 12.0; Pax3Sp-d/Pax3Sp-d, Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS20: skeletal musculature Trace Regionally restricted 7I Expression in a muscle opposite the forelimb is truncated in Pax3Sp/Sp embryos.
Specimen 7J: embryonic day 12.0; Pax3Sp-d/Pax3Sp-d, Pitx2tm2Sac/Pitx2tm2Sac (more )
Structure Level Pattern Image Note
TS20: skeletal musculature Trace Regionally restricted 7J Authors state absence of both genes does not aggravate truncation.
Specimen 7K: embryonic day 12.5; Myf5tm2Pas/Myf5+, Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS20: skeletal musculature Present Regionally restricted 7K Authors report expression in a muscle opposite the forelimb.
Specimen 7L: embryonic day 12.5; Myf5tm2Pas/Myf5tm2Pas, Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS20: skeletal musculature Present Regionally restricted 7L Authors report expression in a muscle opposite the forelimb.
Specimen 7M: embryonic day 12.5; Myf5tm2Pas/Myf5+, Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS20: skeletal musculature Present Regionally restricted 7M Authors report expression in a muscle opposite the forelimb.
Specimen 7N: embryonic day 12.5; Myf5tm2Pas/Myf5tm2Pas, Pitx2tm2Sac/Pitx2tm2Sac (more )
Structure Level Pattern Image Note
TS20: skeletal musculature Trace 7N Double mutants are almost completely devoid of expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
MGI 6.24 		The Jackson Laboratory