Gene Expression Data
Assay Details
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Assay
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Reference:
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J:170532
Pan H, et al., A role for Zic1 and Zic2 in Myf5 regulation and somite myogenesis. Dev Biol. 2011 Mar 1;351(1):120-7
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6274501
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Gene symbol:
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Zic1
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Gene name:
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zinc finger protein of the cerebellum 1
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Probe:
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Zic1 probe5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1B:
embryonic day 9.5
(more  )
Note: 22 somites
Specimen
1B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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22 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: somite |
Present |
Regionally restricted |
1B
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Expression detected definitively in the 6th somite pair anterior to newest formed somite and presomitic mesoderm. No expression in newly formed somites.
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| TS15: unsegmented mesenchyme |
Absent |
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1B
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Specimen
1F:
embryonic day 9.5
(more )
Specimen
1F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube |
Present |
Regionally restricted |
1F
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Expression in dorsal neural tube.
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| TS15: migrating neural crest |
Present |
Regionally restricted |
1F
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In anterior regions, expression in cells between neural tube and dermomyotome, likely migratory neural crest cells.
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Specimen
4A:
embryonic day 9.5
(more )
Specimen
4A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: somite |
Present |
Regionally restricted |
4A
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Expression detected in S6 (6th somite anterior to presomitic mesoderm).
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Specimen
4B:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Zic2tm1Jaru/Zic2tm1Jaru
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: somite |
Present |
Regionally restricted |
4B
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Authors state expression is activated normally in mutant embryos. Expression clearly detected in same somite pair (S6) as in littermate control.
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Specimen
Shh-/- not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: somite |
Present |
Regionally restricted |
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Authors report expression is activated normally in the epaxial somite.
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Specimen
6A:
embryonic day 9.5
(more )
Specimen
6A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Specimen
6B:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nogtm1Amc/Nogtm1Amc
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: somite |
Present |
Regionally restricted |
6B
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Activation of expression in newly formed somites at the 10th somite pair anterior to the presomitic mesoderm.
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Analysis Tools