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Gene Expression Data
Assay Details
Assay
Reference: J:170581 Kwon HJ, et al., Radial glia regulate Cajal-Retzius cell positioning in the early embryonic cerebral cortex. Dev Biol. 2011 Mar 1;351(1):25-34
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6281163
Gene symbol: Nes
Gene name: nestin
Antibody: Rat-401 (MAB353)
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 1A: embryonic day 15.0; Itgb1tm1Mll/? (more )
Note: Double labeled: red - Nes; green - Calb2 (assay MGI:6281165). Controls were either homozygous animals without cre, or heterozygotes with cre.
Structure Level Pattern Image Note
TS23: cerebral cortex Present 1A'' , 1A Expression was detected in radial glia fibers that form a parallel and regularly spaced scaffold that reaches the pia.
TS23: telencephalon pia mater Present 1A , 1A'' Expression was in radial glia endfeet.
Specimen 1B: embryonic day 15.0; Itgb1tm1Mll/Itgb1tm1Mll, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Nes; green - Calb2 (assay MGI:6281165).
Structure Level Pattern Image Note
TS23: cerebral cortex Present 1B'' , 1B Expression was in radial glia fibers that were retracted into deeper cortical areas, leaving a gap near the pia. Ectopic endfeet were labeled in the deeper cortical areas.
Specimen 6A: embryonic day 13.5; Orc3tm1.1Zhua/? (more )
Note: Controls were either homozygous animals without cre, or heterozygotes with cre.
Structure Level Pattern Image Note
TS21: cerebral cortex Present 6A Expression was detected in radial glia fibers in organized scaffolds.
Specimen 6B: embryonic day 13.5; Orc3tm1.1Zhua/Orc3tm1.1Zhua, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: cerebral cortex Present Patchy 6B At E13.5 in the mutant, normal expression was detected in radial glia fibers in organized scaffolds.
Specimen 6C: embryonic day 14.5; Orc3tm1.1Zhua/? (more )
Note: Controls were either homozygous animals without cre, or heterozygotes with cre.
Structure Level Pattern Image Note
TS22: cerebral cortex Present 6C Expression was detected in radial glia fibers in organized scaffolds.
Specimen 6D: embryonic day 14.5; Orc3tm1.1Zhua/Orc3tm1.1Zhua, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: cerebral cortex Present 6D At E14.5 in the mutant cortex, a severe loss of radial glia resulted in large and frequent gaps in the labeled radial glia fiber scaffold.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
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