Pou5f1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:182713 McDonel P, et al., Sin3a is essential for the genome integrity and viability of pluripotent cells. Dev Biol. 2012 Mar 1;363(1):62-73
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6283039
Gene symbol:	Pou5f1
Gene name:	POU domain, class 5, transcription factor 1

Antibody:	anti-Oct3/4 (C-10; sc-5279)
Assay notes:	Fixation with 2.5% paraformaldehyde. Two antibodies were described, but the one used in this assay was not detailed. Anti-Oct-3/4 (N-19) MGI:3702924 may have been used in this assay.

Results

1B 8-cell Oct4 +/+ ©

Specimen 1B Oct4 8-cell +/+: embryonic day 2.5 (more

)

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present		1B 8-cell Oct4 +/+

1B 8-cell Oct4 -/- ©

Specimen 1B Oct4 8-cell -/-: embryonic day 2.5; Sin3a^tm1.1Rdp/Sin3a^tm1.1Rdp (more

)

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present		1B 8-cell Oct4 -/-

1B 16-cell Oct4 +/+ ©

Specimen 1B Oct4 16-cell +/+: embryonic day 3.0 (more

)

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present		1B 16-cell Oct4 +/+

1B 16-cell Oct4 -/- ©

Specimen 1B Oct4 16-cell -/-: embryonic day 3.0; Sin3a^tm1.1Rdp/Sin3a^tm1.1Rdp (more

)

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present		1B 16-cell Oct4 -/-

1B Blastocyst Oct4 +/+ ©

Specimen 1B Oct4 Blastocyst +/+: embryonic day 3.5 (more

)

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		1B Blastocyst Oct4 +/+

1B Blastocyst Oct4 -/- ©

Specimen 1B Oct4 Blastocyst -/-: embryonic day 3.5; Sin3a^tm1.1Rdp/Sin3a^tm1.1Rdp (more

)

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		1B Blastocyst Oct4 -/-

1C Oct4 +/+ ©

Specimen 1C Oct4 +/+: embryonic day 3.5 (more

)

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		1C Oct4 +/+

1C Oct4 -/- ©

Specimen 1C Oct4 -/-: embryonic day 3.5; Sin3a^tm1.1Rdp/Sin3a⁺ (more

)

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		1C Oct4 -/-

2A Oct4 +/- ©

Specimen 2A Oct4 +/-: embryonic day 4.5; Sin3a^tm1.1Rdp/Sin3a⁺ (more

)

Structure	Level	Pattern	Image	Note
TS6: epiblast	Present		2A Oct4 +/-

2A Oct4 -/- ©

Specimen 2A Oct4 -/-: embryonic day 4.5; Sin3a^tm1.1Rdp/Sin3a^tm1.1Rdp (more

)

Structure	Level	Pattern	Image	Note
TS6: epiblast	Ambiguous		2A Oct4 -/-

2B Oct4 +/+ ©

Specimen 2B Oct4 +/+: embryonic day 5.5 (more

)

Structure	Level	Pattern	Image	Note
TS7: epiblast	Present		2B Oct4 +/+

2B Oct4 -/- ©

Specimen 2B Oct4 -/-: embryonic day 5.5; Sin3a^tm1.1Rdp/Sin3a^tm1.1Rdp (more

)

Structure	Level	Pattern	Image	Note
TS7: epiblast	Ambiguous		2B Oct4 -/-

3A Oct4 +/+ ©

Specimen 3A Oct4 +/+: embryonic day 3.5 (more

)

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		3A Oct4 +/+

3A Oct4 -/- ©

Specimen 3A Oct4 -/-: embryonic day 3.5; Sin3a^tm1.1Rdp/Sin3a^tm1.1Rdp (more

)

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		3A Oct4 -/-

3B Oct4 +/+ ©

Specimen 3B Oct4 +/+: embryonic day 4.5 (more

)

Structure	Level	Pattern	Image	Note
TS6: epiblast	Present		3B Oct4 +/+

3B Oct4 -/- ©

Specimen 3B Oct4 -/-: embryonic day 4.5; Sin3a^tm1.1Rdp/Sin3a^tm1.1Rdp (more

)

Structure	Level	Pattern	Image	Note
TS6: epiblast	Ambiguous		3B Oct4 -/-

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