Gene Expression Data
Assay Details
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Assay
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Reference:
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J:182713
McDonel P, et al., Sin3a is essential for the genome integrity and viability of pluripotent cells. Dev Biol. 2012 Mar 1;363(1):62-73
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6283045
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Gene symbol:
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Casp3
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Gene name:
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caspase 3
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Results
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Specimen
3A Caspase3 +/+:
embryonic day 3.5
(more  )
Specimen
3A Caspase3 +/+:
(close  )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Ambiguous |
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3A Caspase 3 +/+
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Authors state the vast majority of wildtype or heterozygous blastocysts had either zero or one expressing cell.
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Specimen
Caspase3 E3.5 +/- not shown:
embryonic day 3.5; Sin3atm1.1Rdp/Sin3a +
(more )
Specimen
Caspase3 E3.5 +/- not shown:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Sin3atm1.1Rdp/Sin3a+
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Ambiguous |
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Authors state the vast majority of wildtype or heterozygous blastocysts had either zero or one expressing cell.
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Specimen
3A Caspase3 -/-:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Sin3atm1.1Rdp/Sin3atm1.1Rdp
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Present |
Single cells |
3A Caspase 3 -/-
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Authors report half of homozygous mutant blastocyst had three of more expressing cells, usually located in the inner cell mass.
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Specimen
3B Caspase3 +/+:
embryonic day 4.5
(more )
Specimen
3B Caspase3 +/+:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 4.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS6: blastocyst |
Ambiguous |
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3B Caspase 3 +/+
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Almost all wild type or heterozygous embryos had zero or one expressing cell.
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Specimen
Caspase3 E4.5 +/- not shown:
embryonic day 4.5; Sin3atm1.1Rdp/Sin3a +
(more )
Specimen
Caspase3 E4.5 +/- not shown:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Sin3atm1.1Rdp/Sin3a+
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Age:
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embryonic day 4.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS6: blastocyst |
Ambiguous |
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Almost all wild type or heterozygous embryos had zero or one expressing cell.
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Specimen
3B Caspase3 -/-:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Sin3atm1.1Rdp/Sin3atm1.1Rdp
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Age:
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embryonic day 4.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS6: blastocyst |
Present |
Single cells |
3B Caspase 3 -/-
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Over 80% of homozygous mutatnt embryos showed 3 or more expressing cells.
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Analysis Tools
