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Gene Expression Data
Assay Details
Assay
Reference: J:200762 Buttler K, et al., Maldevelopment of dermal lymphatics in Wnt5a-knockout-mice. Dev Biol. 2013 Sep 15;381(2):365-76
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6296746
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: anti-CD31
Detection system: Alexa Fluor 488
Results
Specimen 3A/C: postnatal adult (more )
Note: Double labeled: green - CD31 (Pecam1); red - Ror2 (assay MGI:6296748). .
Structure Level Pattern Image Note
TS28: dermis Present Regionally restricted 3A , 3C
TS28: lymphatic vessel Present Regionally restricted 3A , 3C
Specimen 7A/B: embryonic day 18.5 (more )
Note: Triple labeled: green - CD31 (Pecam1); red - Lyve1 (assay MGI:6296750); blue - Dapi.
Structure Level Pattern Image Note
TS26: dermis Present Regionally restricted 7B , 7A Expression in dermis of dorsal thoracic region.
TS26: lymphatic vessel Present Regionally restricted 7A , 7B Co-expression with Lyve1 in numerous small lymphatics.
Specimen 7D/E: embryonic day 18.5; Wnt5atm1Amc/Wnt5atm1Amc (more )
Note: Triple labeled: green - CD31 (Pecam1); red - Lyve1 (assay MGI:6296750); blue - Dapi.
Structure Level Pattern Image Note
TS26: dermis Present Regionally restricted 7E , 7D Expression in dermis of dorsal thoracic region.
TS26: lymphatic vessel Present Regionally restricted 7E , 7D Co-expression with Lyve1 in huge, irregularly distributed lymphatics.
Specimen 7G: embryonic day 18.5; Wnt5atm1Amc/Wnt5atm1Amc (more )
Note: Triple labeled: green - CD31 (Pecam1); red - Prox1 (assay MGI:6296745); blue - Dapi.
Structure Level Pattern Image Note
TS26: dermis Present Regionally restricted 7G Expression in dermis of dorsal thoracic region.
TS26: lymphatic vessel Present Regionally restricted 7G Co-expression with Prox1 in lymphatics with large lumen.
Specimen 8A: embryonic day 18.5; Wnt5atm1Amc/Wnt5a+ (more )
Note: Triple labeled: green - CD31 (Pecam1); red - Lyve1 (assay MGI:6296750); blue - Dapi.
Structure Level Pattern Image Note
TS26: dermis Present Regionally restricted 8A Expression in dermis of abdominal region.
TS26: lymphatic vessel Present Regionally restricted 8A Co-expression with Lyve1 in numberous small lymphatics.
Specimen 8B: embryonic day 18.5; Wnt5atm1Amc/Wnt5atm1Amc (more )
Note: Triple labeled: green - CD31 (Pecam1); red - Lyve1 (assay MGI:6296750); blue - Dapi.
Structure Level Pattern Image Note
TS26: dermis Present Regionally restricted 8B Expression in dermis of abdominal region.
TS26: lymphatic vessel Present Regionally restricted 8B Authors report lymphatics of homozygous mutant mice are larger, but fewer, and located more deeply in the dermis.
Specimen 8C: embryonic day 18.5; Wnt5atm1Amc/Wnt5atm1Amc (more )
Note: Triple labeled: green - CD31 (Pecam1); red - Prox1 (assay MGI:6296745); blue - Dapi.
Structure Level Pattern Image Note
TS26: dermis Present Regionally restricted 8C Expression in dermis of abdominal region.
TS26: lymphatic vessel Present Regionally restricted 8C Expression in cell membrane. Authors report a regular pattern of initial lymphatics in heterozygous mutant embryos.
Specimen 8D: embryonic day 18.5; Wnt5atm1Amc/Wnt5atm1Amc (more )
Note: Triple labeled: green - CD31 (Pecam1); red - Prox1 (assay MGI:6296745); blue - Dapi.
Structure Level Pattern Image Note
TS26: dermis Present Regionally restricted 8D Expression in dermis of abdominal region.
TS26: lymphatic vessel Present Regionally restricted 8D Authors report expression in huge but irregularly distributed lymphatics in homozygous mutant embryos.
Specimen S4B: embryonic day 18.5; Wnt5atm1Amc/Wnt5a+ (more )
Note: Triple labeled: green - CD31 (Pecam1); red - Lyve1 (assay MGI:6296750); blue - Dapi.
Structure Level Pattern Image Note
TS26: dermis Present Regionally restricted S4B
TS26: lymphatic vessel Present Regionally restricted S4B
Specimen S4C: embryonic day 18.5; Wnt5atm1Amc/Wnt5atm1Amc (more )
Note: Triple labeled: green - CD31 (Pecam1); red - Lyve1 (assay MGI:6296750); blue - Dapi.
Structure Level Pattern Image Note
TS26: dermis Present Regionally restricted S4C Authors report no difference between heterozygous and homozygous mutant mice.
TS26: lymphatic vessel Present Regionally restricted S4C Authors report no difference between heterozygous and homozygous mutant mice.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
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