Gene Expression Data
Assay Details
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Assay
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Reference:
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J:218168
Lauing KL, et al., Aggrecan is required for growth plate cytoarchitecture and differentiation. Dev Biol. 2014 Dec 15;396(2):224-36
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6305116
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Gene symbol:
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Ihh
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Gene name:
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Indian hedgehog
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Probe:
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Ihh probe24
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4J:
embryonic day 18.5
(more  )
Specimen
4J:
(close  )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: long bone epiphyseal plate |
Present |
Regionally restricted |
4J
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Expression restricted to prehypertrophic and early hypertrophic chondrocytes.
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| TS26: tibia |
Present |
Regionally restricted |
4J
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Expression restricted to prehypertrophic and early hypertrophic chondrocytes.
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Specimen
4K:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6
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Mutant Allele(s):
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Acancmd-Bc/Acancmd-Bc
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: long bone epiphyseal plate |
Trace |
Regionally restricted |
4K
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Extremely low levels of expression. Single fragmented row of weakly positive chondrocytes.
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| TS26: tibia |
Trace |
Regionally restricted |
4K
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Extremely low levels of expression. Single fragmented row of weakly positive chondrocytes.
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Analysis Tools