Gene Expression Data
Assay Details
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Assay
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Reference:
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J:226923
Bowen CJ, et al., Cadherin-11 coordinates cellular migration and extracellular matrix remodeling during aortic valve maturation. Dev Biol. 2015 Nov 1;407(1):145-57
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6306456
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Gene symbol:
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Sox9
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Gene name:
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SRY (sex determining region Y)-box 9
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Antibody:
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anti-Sox9
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Detection system:
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Alexa Fluor 488
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Results
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Specimen
2D +/+:
embryonic day 18.5
(more  )
Specimen
2D +/+:
(close  )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: aortic valve leaflet |
Strong |
Regionally restricted |
2D +/+
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Prominent cytoplasmic and nuclear expression in endocardium and valve interstitial cells in close proximity. Authors state expression is more highly localized with nuclei in wild type mice.
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Specimen
2D -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Cdh11tm1Mta/Cdh11tm1Mta
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: aortic valve leaflet |
Weak |
Regionally restricted |
2D -/-
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Expression markedly decreased and rarely nuclear. Expression is mostly in cytoplasm.
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Specimen
8C +/+:
postnatal month 10
(more )
Specimen
8C +/+:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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postnatal month 10
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: aortic valve |
Present |
Regionally restricted |
8C +/+
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Expression equally colocalized with nuclei in both wildtype and Cdh11-null mice.
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Specimen
8C -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Cdh11tm1Mta/Cdh11tm1Mta
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Age:
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postnatal month 10
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: aortic valve |
Present |
Regionally restricted |
8C -/-
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Expression equally colocalized with nuclei in both wildtype and Cdh11-null mice.
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Analysis Tools