Gene Expression Data
Assay Details
Assay
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Reference:
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J:78344
Mustonen T, et al., Lunatic fringe, FGF, and BMP regulate the Notch pathway during epithelial morphogenesis of teeth. Dev Biol. 2002 Aug 15;248(2):281-93
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6323241
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Gene symbol:
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Lfng
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Gene name:
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LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
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Probe:
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L-fng probe2
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
Lfng E11 not shown:
embryonic day 11.0
(more )
Specimen
Lfng E11 not shown:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS19: dental placode |
Absent |
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No expression in dental epithelium during initiation of tooth development.
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Specimen
1D:
embryonic day 13.0
(more )
Specimen
1D:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS21: tooth bud |
Absent |
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1D
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No expression in E13 tooth bud.
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Specimen
1E:
embryonic day 13.5
(more )
Specimen
1E:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS21: tooth bud |
Present |
Regionally restricted |
1E
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Expression first seen at late bud stage in epithelial cells at lingual aspect of dental bud flanking the enamel knot.
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Specimen
1F:
embryonic day 14.0
(more )
Specimen
1F:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS22: tooth epithelium |
Present |
Regionally restricted |
1F
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Expression flanks enamel knot on both sides.
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TS22: cervical loop |
Strong |
Regionally restricted |
1F
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Expression also on buccal side and continued with high intensity in lingual cervical loop.
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TS22: stellate reticulum |
Ambiguous |
Regionally restricted |
1F
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No expression in most of stellate reticulum cells.
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