Gene Expression Data
Assay Details
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Assay
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Reference:
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J:84903
Bolcato-Bellemin AL, et al., Laminin alpha5 chain is required for intestinal smooth muscle development. Dev Biol. 2003 Aug 15;260(2):376-90
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6324290
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Gene symbol:
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Lama5
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Gene name:
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laminin, alpha 5
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Antibody:
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anti-laminin alpha5
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Detection system:
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fluorescein
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Assay notes:
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Two antibodies were described, but the one used in this assay was not detailed. Antibody 4G6 MGI:1342262 may have been used in this assay.
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Results
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Specimen
1A:
embryonic day 12.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: intestine |
Present |
Regionally restricted |
1A
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Expression at endodermal/mesenchymal junction and in the serosal layer.
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Specimen
4A:
embryonic day 14.5
(more )
Specimen
4A:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: small intestine mesenchyme |
Present |
Regionally restricted |
4A
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Expression in subepithelial basement membrane. Expression either absent or faint in presumptive muscle cell coat in wild type intestines.
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Specimen
4E:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Lama5tm1Jhm/Lama5tm1Jhm
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: small intestine |
Absent |
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4E
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Authors report expression either absent or faint in mutant intestines.
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Specimen
4I:
embryonic day 16.5
(more )
Specimen
4I:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: small intestine mesenchyme |
Present |
Regionally restricted |
4I
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Expression in subepithelial basement membrane. Expression at periphery in newly formed muscle layers.
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Specimen
4N:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Lama5tm1Jhm/Lama5tm1Jhm
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: small intestine |
Absent |
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4N
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Expression either absent or faint in presumptive muscle cell coat in mutant intestines.
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Analysis Tools