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Gene Expression Data
Assay Details
Assay
Reference: J:170258 Mavromatakis YE, et al., Foxa1 and Foxa2 positively and negatively regulate Shh signalling to specify ventral midbrain progenitor identity. Mech Dev. 2011 Jan-Feb;128(1-2):90-103
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6343569
Gene symbol: Nkx2-2
Gene name: NK2 homeobox 2
Antibody: 74.5A5
Results
Specimen 5A: embryonic day 9.5; Foxa2tm1Khk/Foxa2tm1Khk (more )
Note: Control. Double labeled: green - Nkx2-2; red - Foxa2 (assay MGI:6343553).
Structure Level Pattern Image Note
TS15: future midbrain Present Regionally restricted 5A Expression was detected in the ventral midbrain except at the ventral midline. Expression overlaps with Foxa2.
Specimen 5B: embryonic day 9.5; Foxa2tm1Khk/Foxa2tm1Khk, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. Double labeled: green - Nkx2-2; red - Foxa2 (assay MGI:6343553).
Structure Level Pattern Image Note
TS15: future midbrain Present Regionally restricted 5B The initiation of Nkx2-2 expression in the midbrain occurs normally at E9.5 in the conditional knockout mutant.
Specimen 5C: embryonic day 10.5; Foxa2tm1Khk/Foxa2tm1Khk (more )
Note: Control. Double labeled: green - Nkx2-2; red - Foxa2 (assay MGI:6343553).
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 5C Expression was detected in the ventral midbrain, except at the ventral midline. Co-expression with Foxa2 was progressively becoming restricted to the alar/basal plate boundary.
Specimen 5D: embryonic day 10.5; Foxa2tm1Khk/Foxa2tm1Khk, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. Double labeled: green - Nkx2-2; red - Foxa2 (assay MGI:6343553).
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 5D The normal reduction of Nkx2-2 expression in the ventral midbrain to the alar/basal boundary does not occur in the conditional knockout mutant. The domain of expression is expanded in the basal progenitors.
Specimen 5E: embryonic day 11.5; Foxa2tm1Khk/Foxa2tm1Khk (more )
Note: Control. Double labeled: green - Nkx2-2; red - Foxa2 (assay MGI:6343553).
Structure Level Pattern Image Note
TS19: midbrain Present Regionally restricted 5E Expression was detected in the ventral midbrain, except at the ventral midline. Co-expression with Foxa2 was progressively becoming restricted to the alar/basal plate boundary.
Specimen 5F: embryonic day 11.5; Foxa2tm1Khk/Foxa2tm1Khk, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. Double labeled: green - Nkx2-2; red - Foxa2 (assay MGI:6343553).
Structure Level Pattern Image Note
TS19: midbrain Present Regionally restricted 5F The normal reduction of Nkx2-2 expression in the ventral midbrain to the alar/basal boundary does not occur in the conditional knockout mutant. The domain of expression is expanded in the basal progenitors.
Specimen 5G: embryonic day 12.5; Foxa2tm1Khk/Foxa2tm1Khk (more )
Note: Control. Double labeled: green - Nkx2-2; red - Foxa2 (assay MGI:6343553).
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 5G Expression was detected in the ventral midbrain, except at the ventral midline. Co-expression with Foxa2 was progressively becoming restricted to the alar/basal plate boundary.
Specimen 5H: embryonic day 12.5; Foxa2tm1Khk/Foxa2tm1Khk, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. Double labeled: green - Nkx2-2; red - Foxa2 (assay MGI:6343553).
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 5H The normal reduction of Nkx2-2 expression in the ventral midbrain to the alar/basal boundary does not occur in the conditional knockout mutant. The domain of expression is expanded in the basal progenitors.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/24/2024
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