Results
|
Specimen
1A:
embryonic day 12.5
(more )
Specimen
1A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1B:
(close )
Genetic Background:
|
involves: 129X1/SvJ * C57BL/6 * SJL/J
|
Mutant Allele(s):
|
Smotm2Amc/Smotm2Amc Tg(Prrx1-cre)1Cjt/0
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant.
|
Specimen
1C:
embryonic day 12.5; Smotm2Amc/ Smotm2Amc, Pax3tm1(cre)Joe/Pax3 +
(more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Specimen
1C:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm2Amc/Smotm2Amc Pax3tm1(cre)Joe/Pax3+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: forelimb muscle |
Present |
|
1C
|
The expression domain appeared to be reduced in size at E12.5.
|
Specimen
3M:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm1Amc/Smo+ Pax3tm1(cre)Joe/Pax3+
|
Age:
|
embryonic day 10.75
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud |
Present |
Regionally restricted |
3M
|
Expression was detected in forelimb ventral and dorsal muscle cells.
|
Specimen
3N:
embryonic day 10.75; Smotm1Amc/ Smotm2Amc, Pax3tm1(cre)Joe/Pax3 +
(more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Specimen
3N:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm1Amc/Smotm2Amc Pax3tm1(cre)Joe/Pax3+
|
Age:
|
embryonic day 10.75
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud |
Present |
Regionally restricted |
3N
|
At E10.75, expression was delayed in the conditional knockout ventral muscle cells. Expression in dorsal muscle cells was largely unaffected.
|
Specimen
3Q:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm1Amc/Smo+ Pax3tm1(cre)Joe/Pax3+
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: forelimb bud |
Present |
Regionally restricted |
3Q
|
Expression was detected in forelimb ventral and dorsal muscle cells.
|
Specimen
3R:
embryonic day 11.0; Smotm1Amc/ Smotm2Amc, Pax3tm1(cre)Joe/Pax3 +
(more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Specimen
3R:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm1Amc/Smotm2Amc Pax3tm1(cre)Joe/Pax3+
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: forelimb bud |
Present |
Regionally restricted |
3R
|
Expression in forelimb ventral muscle cells had recovered by E11 in the conditional knockout.
|
Specimen
4A:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm2Amc/Smo+ Pax3tm1(cre)Joe/Pax3+
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: handplate |
Present |
Regionally restricted |
4A
|
Expression was detected in myogenic cells in the ventral autopod.
|
Specimen
4B:
embryonic day 11.5; Smotm2Amc/ Smotm2Amc, Pax3tm1(cre)Joe/Pax3 +
(more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Specimen
4B:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm2Amc/Smotm2Amc Pax3tm1(cre)Joe/Pax3+
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: handplate |
Absent |
|
4B
|
Expression was absent in the distal forelimb.
|
Specimen
4C:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm2Amc/Smo+ Pax3tm1(cre)Joe/Pax3+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hand |
Present |
Regionally restricted |
4C
|
Expression was detected in myogenic cells in the ventral autopod.
|
Specimen
4D:
embryonic day 12.5; Smotm2Amc/ Smotm2Amc, Pax3tm1(cre)Joe/Pax3 +
(more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Specimen
4D:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm2Amc/Smotm2Amc Pax3tm1(cre)Joe/Pax3+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hand |
Absent |
|
4D
|
Expression was absent in the distal forelimb.
|
Specimen
5I:
embryonic day 12.5
(more )
Specimen
5I:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: limb |
Present |
Regionally restricted |
5I
|
Expression was detected in distal myocytes.
|
Specimen
5J:
(close )
Genetic Background:
|
involves: 129S4/SvJaeSor * 129X1/SvJ
|
Mutant Allele(s):
|
Smotm2Amc/Smotm2Amc Myf5tm3(cre)Sor/Myf5+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: limb |
Present |
Regionally restricted |
5J
|
In the conditional knockout using the Myf5 Cre driver, expression didn't extend as far distally as in wild type.
|
Specimen
7K:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm2Amc/Smo+ Pax3tm1(cre)Joe/Pax3+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant.
|
Specimen
7L:
embryonic day 12.5; Smotm2Amc/ Smotm2Amc, Pax3tm1(cre)Joe/Pax3 +
(more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Specimen
7L:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm2Amc/Smotm2Amc Pax3tm1(cre)Joe/Pax3+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: forelimb muscle |
Present |
Regionally restricted |
7L
|
In the conditional knockout mutant, expression didn't extend as far distally in the forelimb muscle as in wild type.
|
Specimen
S1A:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm2Amc/Smo+ Pax3tm1(cre)Joe/Pax3+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant.
|
Specimen
S1B:
embryonic day 12.5; Smotm2Amc/ Smotm2Amc, Pax3tm1(cre)Joe/Pax3 +
(more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Specimen
S1B:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm2Amc/Smotm2Amc Pax3tm1(cre)Joe/Pax3+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: somite |
Present |
Regionally restricted |
S1B
|
In the conditional knockout mutant, expression was lost in the cervical somites.
|
Specimen
S1E:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm2Amc/Smo+ Tcf7l2tm1(EGFP/cre)Mrc/Tcf7l2+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant.
|
Specimen
S1F:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm2Amc/Smotm2Amc Tcf7l2tm1(EGFP/cre)Mrc/Tcf7l2+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: limb muscle |
Present |
|
S1F
|
Expression in the conditional knockout mutant using the Tcf4-Cre driver appeared normal.
|
Specimen
S4M:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm1Amc/Smo+ Pax3tm1(cre)Joe/Pax3+
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: hindlimb bud |
Present |
Regionally restricted |
S4M
|
Expression was detected in hindlimb ventral and dorsal muscle cells.
|
Specimen
S4N:
embryonic day 11.0; Smotm1Amc/ Smotm2Amc, Pax3tm1(cre)Joe/Pax3 +
(more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Specimen
S4N:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm1Amc/Smotm2Amc Pax3tm1(cre)Joe/Pax3+
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: hindlimb bud |
Absent |
|
S4N
|
At E11, expression was delayed in the conditional knockout ventral and dorsal muscle cells.
|
Specimen
S4O:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm1Amc/Smo+ Pax3tm1(cre)Joe/Pax3+
|
Age:
|
embryonic day 11.25
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: hindlimb bud |
Present |
Regionally restricted |
S4O
|
Expression was detected in hindlimb ventral and dorsal muscle cells.
|
Specimen
S4P:
embryonic day 11.25; Smotm1Amc/ Smotm2Amc, Pax3tm1(cre)Joe/Pax3 +
(more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Specimen
S4P:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm1Amc/Smotm2Amc Pax3tm1(cre)Joe/Pax3+
|
Age:
|
embryonic day 11.25
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: hindlimb bud |
Present |
Regionally restricted |
S4P
|
Expression in hindlimb ventral and dorsal muscle cells had recovered by E11.25 in the conditional knockout.
|
Specimen
S5A:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm2Amc/Smo+ Pax3tm1(cre)Joe/Pax3+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: foot |
Present |
Regionally restricted |
S5A
|
Expression was detected in myogenic cells in the ventral hindlimb.
|
Specimen
S5B:
embryonic day 12.5; Smotm2Amc/ Smotm2Amc, Pax3tm1(cre)Joe/Pax3 +
(more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Specimen
S5B:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm2Amc/Smotm2Amc Pax3tm1(cre)Joe/Pax3+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: foot |
Weak |
Regionally restricted |
S5B
|
There was a reduced myogenic population in the conditional knockout mutant hindlimb autopod.
|
Specimen
S7E:
embryonic day 13.5
(more )
Specimen
S7E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: autopod |
Present |
Regionally restricted |
S7E
|
Expression was detected in autopod muscles.
|
Specimen
S7F:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Smotm2Amc/Smotm2Amc Tg(MYOD1-cre)M23Glh/0
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: autopod |
Present |
Regionally restricted |
S7F
|
Expression was truncated distally in conditional knockout autopod muscles.
|
|