Acta2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:187741 Hu JK, et al., Autonomous and nonautonomous roles of Hedgehog signaling in regulating limb muscle formation. Genes Dev. 2012 Sep 15;26(18):2088-102
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6358453
Gene symbol:	Acta2
Gene name:	actin alpha 2, smooth muscle, aorta

Antibody:

Results

1D ©

Specimen 1D: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: forelimb muscle	Present		1D

1E ©

Specimen 1E: embryonic day 16.5; Smo^tm2Amc/Smo^tm2Amc, Tg(Prrx1-cre)1Cjt/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS24: forelimb muscle	Present		1E

1F ©

Specimen 1F: embryonic day 16.5; Smo^tm2Amc/Smo^tm2Amc, Pax3^tm1(cre)Joe/Pax3⁺ (more

)
Note: Conditional mutant. Pax3^Cre;Smo^CKO specimens can be either Pax3^Cre;Smo^fl/fl or Pax3^Cre;Smo^fl/del.

Structure	Level	Pattern	Image	Note
TS24: forelimb muscle	Present		1F

4K ©

Specimen 4K: embryonic day 16.5; Smo^tm2Amc/Smo⁺, Pax3^tm1(cre)Joe/Pax3⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS24: hand muscle	Present		4K
TS24: hand	Present	Regionally restricted	4K	Expression was detected in muscles and endothelial cells.

4L ©

Specimen 4L: embryonic day 16.5; Smo^tm2Amc/Smo^tm2Amc, Pax3^tm1(cre)Joe/Pax3⁺ (more

)
Note: Conditional mutant. Pax3^Cre;Smo^CKO specimens can be either Pax3^Cre;Smo^fl/fl or Pax3^Cre;Smo^fl/del.

Structure	Level	Pattern	Image	Note
TS24: hand muscle	Absent		4L
TS24: hand	Present	Regionally restricted	4L	Expression was detected normally in endothelial cells in the mutant autopod.

S2A ©

S2A" ©

S2B ©

S2B" ©

Specimen S2A,B: embryonic day 16.5 (more

)
Note: Double labeled: green - Acta2; red - Laminin.

Structure	Level	Pattern	Image	Note
TS24: forearm muscle	Present		S2A , S2A" , S2B , S2B"	Expression was detected in skeletal muscle bundles in forelimbs at the zeugopod level and colocalized with Laminin.

S5C ©

Specimen S5C: embryonic day 16.5; Smo^tm2Amc/Smo⁺, Pax3^tm1(cre)Joe/Pax3⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS24: blood vessel	Present		S5C
TS24: foot muscle	Present		S5C

S5D ©

Specimen S5D: embryonic day 16.5; Smo^tm2Amc/Smo^tm2Amc, Pax3^tm1(cre)Joe/Pax3⁺ (more

)
Note: Conditional mutant. Pax3^Cre;Smo^CKO specimens can be either Pax3^Cre;Smo^fl/fl or Pax3^Cre;Smo^fl/del.

Structure	Level	Pattern	Image	Note
TS24: foot muscle	Present		S5D	There was a partial loss of muscles in the conditional knockout mutant feet.
TS24: blood vessel	Present		S5D	Blood vessels were unaffected in the mutant.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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