Gene Expression Data
Assay Details
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Assay
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Reference:
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J:187741
Hu JK, et al., Autonomous and nonautonomous roles of Hedgehog signaling in regulating limb muscle formation. Genes Dev. 2012 Sep 15;26(18):2088-102
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6358468
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Gene symbol:
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Myf5
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Gene name:
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myogenic factor 5
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Probe:
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Myf5 probe25
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3C:
(close  )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Smotm1Amc/Smo+
Pax3tm1(cre)Joe/Pax3+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
3C
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Expression was detected in forelimb ventral and dorsal muscle cells.
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Specimen
3D:
embryonic day 10.5; Smotm1Amc/ Smotm2Amc, Pax3tm1(cre)Joe/Pax3 +
(more  )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Specimen
3D:
(close )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Smotm1Amc/Smotm2Amc
Pax3tm1(cre)Joe/Pax3+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
3D
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At E10.5, expression was delayed in the conditional knockout ventral muscle cells. Expression in the dorsal muscle cells was largely unaffected.
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Specimen
3I:
(close )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Smotm1Amc/Smo+
Pax3tm1(cre)Joe/Pax3+
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Age:
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embryonic day 10.75
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
3I
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Expression was detected in forelimb ventral and dorsal muscle cells.
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Specimen
3J:
embryonic day 10.75; Smotm1Amc/ Smotm2Amc, Pax3tm1(cre)Joe/Pax3 +
(more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Specimen
3J:
(close )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Smotm1Amc/Smotm2Amc
Pax3tm1(cre)Joe/Pax3+
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Age:
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embryonic day 10.75
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
3J
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Expression in forelimb ventral muscle cells had recovered by E10.75 in the conditional knockout.
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Specimen
S4C:
(close )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Smotm1Amc/Smo+
Pax3tm1(cre)Joe/Pax3+
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Age:
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embryonic day 10.75
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
S4C
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Expression was detected in hindlimb ventral and dorsal muscle cells.
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Specimen
S4D:
embryonic day 10.75; Smotm1Amc/ Smotm2Amc, Pax3tm1(cre)Joe/Pax3 +
(more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Specimen
S4D:
(close )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Smotm1Amc/Smotm2Amc
Pax3tm1(cre)Joe/Pax3+
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Age:
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embryonic day 10.75
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
S4D
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At E10.75, expression was delayed in the conditional knockout ventral muscle cells. Expression in the dorsal muscle cells was largely unaffected.
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Specimen
S4I:
(close )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Smotm1Amc/Smo+
Pax3tm1(cre)Joe/Pax3+
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: hindlimb bud |
Present |
Regionally restricted |
S4I
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Expression was detected in hindlimb ventral and dorsal muscle cells.
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Specimen
S4J:
embryonic day 11.0; Smotm1Amc/ Smotm2Amc, Pax3tm1(cre)Joe/Pax3 +
(more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Specimen
S4J:
(close )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Smotm1Amc/Smotm2Amc
Pax3tm1(cre)Joe/Pax3+
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: hindlimb bud |
Present |
Regionally restricted |
S4J
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Expression in hindlimb ventral muscle cells had recovered by E11 in the conditional knockout.
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Analysis Tools