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Gene Expression Data
Assay Details
Assay
Reference: J:187741 Hu JK, et al., Autonomous and nonautonomous roles of Hedgehog signaling in regulating limb muscle formation. Genes Dev. 2012 Sep 15;26(18):2088-102
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6358479
Gene symbol: Myf5
Gene name: myogenic factor 5
Antibody: Anti-Myf-5 (C-20)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 3E: embryonic day 10.5; Smotm1Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Double labeled: red - Myf5; green - Pax3 (assay MGI:6358478).
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 3E Expression was detected in forelimb ventral and dorsal muscle cells.
Specimen 3F: embryonic day 10.5; Smotm1Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del. Double labeled: red - Myf5; green - Pax3 (assay MGI:6358478).
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 3F At E10.5, expression was delayed in the conditional knockout ventral muscle cells. Expression in the dorsal muscle cells was largely unaffected.
Specimen 3K: embryonic day 10.75; Smotm1Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Double labeled: red - Myf5; green - Pax3 (assay MGI:6358478).
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 3K Expression was detected in forelimb ventral and dorsal muscle cells.
Specimen 3L: embryonic day 10.75; Smotm1Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del. Double labeled: red - Myf5; green - Pax3 (assay MGI:6358478).
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 3L Expression in forelimb ventral muscle cells had recovered by E10.75 in the conditional knockout.
Specimen S4E: embryonic day 10.75; Smotm1Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Double labeled: red - Myf5; green - Pax3 (assay MGI:6358478).
Structure Level Pattern Image Note
TS17: hindlimb bud Present Regionally restricted S4E Expression was detected in hindlimb ventral and dorsal muscle cells.
Specimen S4F: embryonic day 10.75; Smotm1Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del. Double labeled: red - Myf5; green - Pax3 (assay MGI:6358478).
Structure Level Pattern Image Note
TS17: hindlimb bud Present Regionally restricted S4F At E10.75, expression was delayed in the conditional knockout ventral muscle cells. Expression in the dorsal muscle cells was largely unaffected.
Specimen S4K: embryonic day 11.0; Smotm1Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Double labeled: red - Myf5; green - Pax3 (assay MGI:6358478).
Structure Level Pattern Image Note
TS18: hindlimb bud Present Regionally restricted S4K Expression was detected in hindlimb ventral and dorsal muscle cells.
Specimen S4L: embryonic day 11.0; Smotm1Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del. Double labeled: red - Myf5; green - Pax3 (assay MGI:6358478).
Structure Level Pattern Image Note
TS18: hindlimb bud Present Regionally restricted S4L Expression in hindlimb ventral muscle cells had recovered by E11 in the conditional knockout.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
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