Probe:	NX2a
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	3C	Expression in the thoracic level was more restricted than at the lumbar level. Expression was in postmitotic neurons.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present		3H	Expression was in postmitotic neurons.
TS20: dorsal root ganglion	Absent		3H

Structure	Level	Pattern	Image	Note
TS23: spinal cord gray matter	Strong	Uniform	3M

Structure	Level	Pattern	Image	Note
TS23: dorsal root ganglion	Present	Regionally restricted	4E , 4F	Expression was detected in a subset of neurons.
TS23: sympathetic ganglion	Absent		4E

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Widespread	5C	Expression was detected in all parts of the brain with the exception of the forebrain, where only weak signals were seen. Expression was restricted to postmitotic neurons that had left the ventricular zone.
TS20: forebrain	Weak		5C

Structure	Level	Pattern	Image	Note
TS23: brain	Present	Widespread	5H	Expression was detected in most brain regions.
TS23: cervical ganglion	Present		5H
TS23: trigeminal ganglion	Present		5H

Structure	Level	Pattern	Image	Note
TS23: cerebellum	Strong		5M
TS23: cortical plate	Strong		5M
TS23: hindbrain	Strong		5M
TS23: hippocampus	Strong		5M
TS23: midbrain	Strong		5M
TS23: tectum	Strong	Non-Uniform	5M	The anterior parts of the tectum displayed higher levels of transcript than the more posterior parts.

Structure	Level	Pattern	Image	Note
TS23: trigeminal ganglion	Present		6C

Structure	Level	Pattern	Image	Note
TS23: retina nuclear layer	Present	Regionally restricted		Expression was detected in all cells of the inner nuclear layer.