Probe:	NX3a
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	3E	Expression in the thoracic level was more restricted than at the lumbar level. Expression was in postmitotic neurons.

Structure	Level	Pattern	Image	Note
TS20: spinal cord mantle layer	Strong	Regionally restricted	3J	Strong expression was detected in the more medial part of the mantle zone.
TS20: spinal cord	Present	Regionally restricted	3J	Expression was strong in the medial part of the mantle zone, but the intermediate zone and the most ventral part of the spinal cord were not labeled. Expression was in postmitotic neurons.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted		Results were similar to the caudal region of E12 embryos.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted		Results were similar to the caudal region of E12 embryos.

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Present	Scattered	3O	Expression was detected in a subset of neurons that were uniformly scattered throughout the spinal cord.
TS23: dorsal root ganglion	Absent		3O
TS23: sympathetic ganglion	Absent		3O

Structure	Level	Pattern	Image	Note
TS21: forebrain-midbrain boundary region	Strong		5E
TS21: ganglionic eminence	Strong		5E
TS21: midbrain tegmentum	Strong		5E
TS21: midbrain roof plate	Absent		5E

Structure	Level	Pattern	Image	Note
TS23: caudate-putamen	Strong		5J
TS23: corpus striatum	Strong		5J
TS23: cerebral cortex	Strong	Regionally restricted	5J	Prominent expression was detected in some restricted cell populations.
TS23: hindbrain	Strong	Regionally restricted	5J	Prominent expression was detected in some restricted cell populations.
TS23: midbrain	Strong	Regionally restricted	5J	Prominent expression was detected in some restricted cell populations.

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Present	Regionally restricted	5O	Expression was seen in two distinct cell layers in the cortical plate.
TS23: telencephalon	Present	Regionally restricted	5O	Expression was detected at the interface between the cortical neuroepithelium and the corpus callosum.
TS23: cervical ganglion	Ambiguous		5O	Expression was absent or only weakly expressed in cervical ganglia.

Structure	Level	Pattern	Image	Note
TS23: retina nuclear layer	Present	Regionally restricted	7B	Expression was restricted to a small zone of the inner nuclear layer adjacent to the outer nuclear layer.

Structure	Level	Pattern	Image	Note
TS23: face	Present	Regionally restricted	7D	Expression was detected around the primordia of the vibrissa follicles.