Gene Expression Data
Assay Details
Assay
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Reference:
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J:279271
Ellis K, et al., GSK3 regulates hair cell fate in the developing mammalian cochlea. Dev Biol. 2019 Sep 15;453(2):191-205
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6364592
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Gene symbol:
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Gsk3b
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Gene name:
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glycogen synthase kinase 3 beta
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Results
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Specimen
2A'/2A":
embryonic day 14.5
(more )
Note: Double labeled: pink - Gsk3b; green - F-actin.
Specimen
2A'/2A":
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: pink - Gsk3b; green - F-actin.
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Structure |
Level |
Pattern |
Image |
Note |
TS22: cochlea |
Present |
Regionally restricted |
2A'
,
2A"
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Expression in single row of cells located near medio-lateral border of organ of Corti, just lateral to developing inner hair cells. Authors state cells located in this position will develop as inner pllar cells. Lower levels of expression in developing outer hair cells region. Similar levels of expression in cells located lateral to the sensory epithelium. Authors state these cells will develop as Hensen's and Claudius cells and the outer sulcus.
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Specimen
2B/2B':
embryonic day 14.5
(more )
Note: Double labeled: pink - Gsk3b; green - Myo7a (assay MGI:6364593).
Specimen
2B/2B':
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
|
Note:
|
Double labeled: pink - Gsk3b; green - Myo7a (assay MGI:6364593).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: cochlea |
Present |
Regionally restricted |
2B'
,
2B
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Expression at apical surface of epithelium. Peak intensity of expression corresponds to inner pillar cells and Hensen's/Claudius/outer sulcus cells.
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Specimen
2C'/2C":
embryonic day 18.5
(more )
Note: Double labeled: pink - Gsk3b; green - F-actin.
Specimen
2C'/2C":
(close )
Genetic Background:
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CD-1
|
Age:
|
embryonic day 18.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: pink - Gsk3b; green - F-actin.
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: organ of Corti |
Present |
Regionally restricted |
2C"
,
2C'
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Medial expression in inner pillar cells. Weaker expression in outer pillar cells, inner phalangeal cells and border cells. More intense expression in restricted group of cells lateral to sensory epithelium. Expression in Hensen's and Claudius cells.
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Specimen
2D/2D':
embryonic day 18.5
(more )
Note: Double labeled: pink - Gsk3b; green - Myo7a (assay MGI:6364593).
Specimen
2D/2D':
(close )
Genetic Background:
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CD-1
|
Age:
|
embryonic day 18.5
|
Sex:
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Not Specified
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Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: pink - Gsk3b; green - Myo7a (assay MGI:6364593).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: organ of Corti |
Present |
Regionally restricted |
2D'
,
2D
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Peak intensity of expression corresponds to inner pilar cells and Hensen's/Claudius cells.
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Specimen
2E'/2E":
postnatal day 0
(more )
Note: Double labeled: pink - Gsk3b; green - F-actin.
Specimen
2E'/2E":
(close )
Genetic Background:
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CD-1
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
|
Double labeled: pink - Gsk3b; green - F-actin.
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Structure |
Level |
Pattern |
Image |
Note |
TS27: organ of Corti |
Present |
Regionally restricted |
2E"
,
2E'
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Expression markedly decreased overall but detected in inner pillar cells, Hensen's and Claudius cells.
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Specimen
2F/2F':
postnatal day 0
(more )
Note: Double labeled: pink - Gsk3b; green - Myo7a (assay MGI:6364593).
Specimen
2F/2F':
(close )
Genetic Background:
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CD-1
|
Age:
|
postnatal day 0
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Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: pink - Gsk3b; green - Myo7a (assay MGI:6364593).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: organ of Corti |
Present |
Regionally restricted |
2F
,
2F'
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Expression at apical surface of epithelium.
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