Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:119875
Lebel M, et al., Gli2 and Gli3 play distinct roles in the dorsoventral patterning of the mouse hindbrain. Dev Biol. 2007 Feb 1;302(1):345-55
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:6368136
|
|
Gene symbol:
|
Olig2
|
|
Gene name:
|
oligodendrocyte transcription factor 2
|
|
Probe:
|
Olig2 probe7
|
|
Probe preparation:
|
labelled with digoxigenin RNA
|
|
|
Results
|
Specimen
6A:
embryonic day 10.5
(more  )
Note: After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
Specimen
6A:
(close  )
|
Genetic Background:
|
involves: CD-1
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
6A
|
Expression mostly restricted to rhombomeres 5 and 7, and spinal cord.
|
| TS17: rhombomere 5 |
Present |
Regionally restricted |
6A
|
Expression mostly restricted to rhombomeres 5 and 7, and spinal cord.
|
| TS17: rhombomere 7 |
Present |
Regionally restricted |
6A
|
Expression mostly restricted to rhombomeres 5 and 7, and spinal cord.
|
| TS17: rhombomere 4 |
Weak |
Regionally restricted |
6A
|
Lower levels of expression in oligodendrocyte precursors in rhombomeres 4 and 6.
|
| TS17: rhombomere 6 |
Weak |
Regionally restricted |
6A
|
Lower levels of expression in oligodendrocyte precursors in rhombomeres 4 and 6.
|
Specimen
6B:
embryonic day 10.5; Gli2tm1Alj/ Gli2tm1Alj
(more )
Note: After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
Specimen
6B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * CD-1
|
|
Mutant Allele(s):
|
Gli2tm1Alj/Gli2tm1Alj
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: rhombomere 7 |
Present |
Regionally restricted |
6B
|
Expression slightly more ventral in rhombomere 7
|
| TS17: rhombomere 5 |
Present |
Regionally restricted |
6B
|
Expression eliminated in anterior part of rhombomere 5.
|
Specimen
6C:
embryonic day 10.5; Gli3Xt-J/ Gli3Xt-J
(more )
Note: After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
Specimen
6C:
(close )
|
Genetic Background:
|
involves: C3H/HeJ * CD-1
|
|
Mutant Allele(s):
|
Gli3Xt-J/Gli3Xt-J
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
6C
|
Expression not significanly affected compared to wildtype.
|
|
Analysis Tools