Gene Expression Data
Assay Details
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Assay
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Reference:
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J:39350
Schneider-Maunoury S, et al., Segmental and neuronal architecture of the hindbrain of Krox-20 mouse mutants. Development. 1997 Mar;124(6):1215-26
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6376239
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Gene symbol:
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Egr2
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Gene name:
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early growth response 2
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
1A:
embryonic day 9.5; Egr2tm1Pch/Egr2 +
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
1B:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2tm1Pch
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: rhombomere |
Weak |
Regionally restricted |
1B
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In the homozygous mutant embryo, staining is restricted to a small region of the alar plate which presumably corresponds to remnants of rhombomere 5.
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Specimen
2E:
embryonic day 10.5; Egr2tm1Pch/Egr2 +
(more )
Note: Double labeled: blue - Egr2; purple - Lhx1 (assay MGI:6376253).
Specimen
2E:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Double labeled: blue - Egr2; purple - Lhx1 (assay MGI:6376253).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: rhombomere 5 |
Present |
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2E
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Beta-galactosidase staining in rhombomere 5 was flanked by two transverse Lhx1-positive bands, which correspond to the r4/r5 and r5/r6 rhombomere boundaries. The blue spots of X-gal staining rostral and caudal to the rhombomere 5 staining were remnants of the facial and glossopharyngeal roots.
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Analysis Tools