Antibody:	anti-neurofilament clone 2H3
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS15: rhombomere	Present	Non-Uniform	1A	Rhombomeres 3 and 5 were distinguished from even-numbered rhombomeres by lower immunoreactivity. Two regions of low immunoreactivity that overlapped with beta-galactosidase staining were noted in the control embryo.

Structure	Level	Pattern	Image	Note
TS15: rhombomere	Present	Non-Uniform	1B	In the homozygous mutant, only one region of lower immunoreactivity was noted in the region of rhombomere 6. The staining between presumptive rhombomeres 2 and 4 was continuous.

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present	Regionally restricted	3A	Expression was detected in the Vth and VIIth motor roots, which exit the hindbrain and enter the first and second branchial arches, respectively.

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present	Regionally restricted	3B	The immunopositive Vth and VIIth motor roots exit the brain at separate points but fasciculate and enter the second branchial arch as a single nerve in the homozygous mutant.