Wnt3a RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:122556 Inoue T, et al., Zic2 and Zic3 synergistically control neurulation and segmentation of paraxial mesoderm in mouse embryo. Dev Biol. 2007 Jun 15;306(2):669-84
Assay type:	RNA in situ
MGI Accession ID:	MGI:6377223
Gene symbol:	Wnt3a
Gene name:	wingless-type MMTV integration site family, member 3A

Probe:	Wnt3a probe2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

9D ©

Specimen 9D: embryonic day 8.5 (more

)
Note: Also stained for Otx2 (assay MGI:6377219).

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present		9D

9E ©

Specimen 9E: embryonic day 8.5; Zic3^Bn/Y (more

)
Note: Also stained for Otx2 (assay MGI:6377219).

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present		9E

9F (left) ©

Specimen 9F (left): embryonic day 8.5; Zic2^tm1Jaru/Zic2^tm1Jaru, Zic3^Bn/Y (more

)
Note: Also stained for Otx2 (assay MGI:6377219).

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Weak	Regionally restricted	9F (left)	Expression generally reduced in size, and in intensity in 43% of kd/+ Bn/Y mutants.

9F (right) ©

Specimen 9F (right): embryonic day 8.5; Zic2^tm1Jaru/Zic2^tm1Jaru, Zic3^Bn/Y (more

)
Note: Also stained for Otx2 (assay MGI:6377219).

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Weak	Regionally restricted	9F (right)	Expression generally reduced in size, and in intensity in 43% of kd/+ Bn/Y mutants.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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