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Gene Expression Data
Assay Details
Assay
Reference: J:275243 Bosch PJ, et al., A critical role for the nuclear protein Akirin2 in the formation of mammalian muscle in vivo. Genesis. 2019 May;57(5):e23286
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6379246
Gene symbol: Pax7
Gene name: paired box 7
Antibody: anti-Pax7
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3b Sim1-Cre;Aki2fl/+: embryonic day 14.5; Akirin2tm1Aki/Akirin2+, Tg(Sim1-cre)1Lowl/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: intercostal muscle Present 3b Pax7 Sim1-Cre;Aki2fl/+
Specimen 3b Sim1-Cre;Aki2fl/fl: embryonic day 14.5; Akirin2tm1Aki/Akirin2tm1Aki, Tg(Sim1-cre)1Lowl/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: intercostal muscle Weak 3b Pax7 Sim1-Cre;Aki2fl/fl There was a reduction of immunostaining between individual ribs in mutants.
Specimen 4c Sim1-Cre;Aki2fl/+: embryonic day 11.5; Akirin2tm1Aki/Akirin2+, Tg(Sim1-cre)1Lowl/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: forelimb bud Present 4c Pax7 Sim1-Cre;Aki2fl/+
TS19: trunk Present 4c Pax7 Sim1-Cre;Aki2fl/+
Specimen 4c Sim1-Cre;Aki2fl/fl: embryonic day 11.5; Akirin2tm1Aki/Akirin2tm1Aki, Tg(Sim1-cre)1Lowl/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: forelimb bud Absent 4c Pax7 Sim1-Cre;Aki2fl/fl
TS19: trunk Present Regionally restricted 4c Pax7 Sim1-Cre;Aki2fl/fl A population of immunopositive epaxial cells remain in the body.
Specimen 4d Sim1-Cre;Aki2fl/+: embryonic day 14.5; Akirin2tm1Aki/Akirin2+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Sim1-cre)1Lowl/0 (more )
Note: Conditional mutant. Double labeled: green - Pax7; red - Cre reporter.
Structure Level Pattern Image Note
TS22: forelimb Present Regionally restricted 4d Merge (Pax7) Sim1-Cre;Aki2fl/+ , 4d Pax7 Sim1-Cre;Aki2fl/+ Expression was detected in myogenic pregenitors.
Specimen 4d Sim1-Cre;Aki2fl/fl: embryonic day 14.5; Akirin2tm1Aki/Akirin2tm1Aki, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Sim1-cre)1Lowl/0 (more )
Note: Conditional mutant. Double labeled: green - Pax7; red - Cre reporter.
Structure Level Pattern Image Note
TS22: forelimb Absent 4d Pax7 Sim1-Cre;Aki2fl/fl , 4d Merge (Pax7) Sim1-Cre;Aki2fl/fl
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
MGI 6.24 		The Jackson Laboratory