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Gene Expression Data
Assay Details
Assay
Reference: J:272586 Suzuki T, et al., Postnatal liver functional maturation requires Cnot complex-mediated decay of mRNAs encoding cell cycle and immature liver genes. Development. 2019 Feb 15;146(4):dev168146
Assay type: RT-PCR
MGI Accession ID: MGI:6383157
Gene symbol: H19
Gene name: H19, imprinted maternally expressed transcript
Probe: H19-pY1, H19-pZ1
Assay notes: Quantitative RT-PCR. Gapdh mRNA levels were used for normalization. Expression levels of genes in postnatal week 8 control mice were set to 1.
Results
Image: 5D H19 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex Note
E16.5 Control E16.5 TS24: liver Present 1 µg; total RNA involves: 129P2/OlaHsd Not Specified The following genotypes could have been used as control samples: (flox/+), (flox/flox), Alb-cre;(+/+) or Alb-cre;(flox/+).
E16.5 Cnot3LKO E16.5 TS24: liver Present (b) 1 µg; total RNA involves: 129P2/OlaHsd * C57BL/6 * DBA Cnot3tm1.2Tya/Cnot3tm1.2Tya, Speer6-ps1Tg(Alb-cre)21Mgn/0Not Specified Conditional mutant.
4w Control P w 4 TS28: liver Present 1 µg; total RNA involves: 129P2/OlaHsd Not Specified The following genotypes could have been used as control samples: (flox/+), (flox/flox), Alb-cre;(+/+) or Alb-cre;(flox/+).
4w Cnot3LKO P w 4 TS28: liver Present (c) 1 µg; total RNA involves: 129P2/OlaHsd * C57BL/6 * DBA Cnot3tm1.2Tya/Cnot3tm1.2Tya, Speer6-ps1Tg(Alb-cre)21Mgn/0Not Specified Conditional mutant.
8w Control P w 8 TS28: liver Present 1 µg; total RNA involves: 129P2/OlaHsd Not Specified The following genotypes could have been used as control samples: (flox/+), (flox/flox), Alb-cre;(+/+) or Alb-cre;(flox/+).
8w Cnot3LKO P w 8 TS28: liver Present (c) 1 µg; total RNA involves: 129P2/OlaHsd * C57BL/6 * DBA Cnot3tm1.2Tya/Cnot3tm1.2Tya, Speer6-ps1Tg(Alb-cre)21Mgn/0Not Specified Conditional mutant.
Notes:
(a) Expression was detected at high levels in immature liver and decreases as the liver develops.
(b) In the embryonic liver, expression was comparable between control and Cnot3LKO liver.
(c) Transcripts were upregulated in the conditional mutant liver compared with control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
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