Gene Expression Data
Assay Details
Assay
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Reference:
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J:234935
Meng XQ, et al., Subcellular localization of proline-rich tyrosine kinase 2 during oocyte fertilization and early-embryo development in mice. J Reprod Dev. 2016 Aug 25;62(4):351-8
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6383975
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Gene symbol:
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Ptk2b
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Gene name:
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PTK2 protein tyrosine kinase 2 beta
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Results
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Specimen
3A2/A:
embryonic day 0.5
(more )
Note: Double labeled: green - Ptk2b; red - propidium iodide (PI, for DNA).
Specimen
3A2/A:
(close )
Genetic Background:
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Kunming
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Age:
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embryonic day 0.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Ptk2b; red - propidium iodide (PI, for DNA).
|
Structure |
Level |
Pattern |
Image |
Note |
TS1: 1-cell stage embryo |
Present |
Regionally restricted |
3A2/A
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Expression in nucleus and cytoplasm. Expression in cytoplasm not evenly distributed, but aggregated as many tiny spots.
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Specimen
3B2/B:
embryonic day 1.0
(more )
Note: Double labeled: green - Ptk2b; red - propidium iodide (PI, for DNA).
Specimen
3B2/B:
(close )
Genetic Background:
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Kunming
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Age:
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embryonic day 1.0
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
|
4% Paraformaldehyde
|
Note:
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Double labeled: green - Ptk2b; red - propidium iodide (PI, for DNA).
|
Structure |
Level |
Pattern |
Image |
Note |
TS2: 2-cell stage embryo |
Present |
Regionally restricted |
3B2/B
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Expression in nucleus and cytoplasm. Expression in cytoplasm not evenly distributed, but aggregated as many tiny spots.Expression also detected in cell junctions.
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Specimen
3C2/C:
embryonic day 2.0
(more )
Note: Double labeled: green - Ptk2b; red - propidium iodide (PI, for DNA).
Specimen
3C2/C:
(close )
Genetic Background:
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Kunming
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Age:
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embryonic day 2.0
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Sex:
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Not Specified
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Type:
|
optical section
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Ptk2b; red - propidium iodide (PI, for DNA).
|
Structure |
Level |
Pattern |
Image |
Note |
TS3: 4-cell stage embryo |
Present |
Regionally restricted |
3C2/C
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Expression in nucleus and cytoplasm. Expression uniformly distributed throughout cytoplasm.
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Specimen
3D2/D:
embryonic day 2.5
(more )
Note: Double labeled: green - Ptk2b; red - propidium iodide (PI, for DNA).
Specimen
3D2/D:
(close )
Genetic Background:
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Kunming
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Age:
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embryonic day 2.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Ptk2b; red - propidium iodide (PI, for DNA).
|
Structure |
Level |
Pattern |
Image |
Note |
TS3: 8-cell stage embryo |
Present |
Regionally restricted |
3D2/D
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Expression in nucleus and cytoplasm. Expression in cytoplasm not evenly distributed, but aggregated as many tiny spots.
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Specimen
3E2/E:
embryonic day 3.0
(more )
Note: Double labeled: green - Ptk2b; red - propidium iodide (PI, for DNA).
Specimen
3E2/E:
(close )
Genetic Background:
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Kunming
|
Age:
|
embryonic day 3.0
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Sex:
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Not Specified
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Type:
|
optical section
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Fixation:
|
4% Paraformaldehyde
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Note:
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Double labeled: green - Ptk2b; red - propidium iodide (PI, for DNA).
|
Structure |
Level |
Pattern |
Image |
Note |
TS3: compacted morula |
Present |
Regionally restricted |
3E2/E
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Expression in nucleus and cytoplasm. Expression in cytoplasm not evenly distributed, but aggregated as many tiny spots.
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Specimen
3F2/F:
embryonic day 3.0
(more )
Note: Double labeled: green - Ptk2b; red - propidium iodide (PI, for DNA).
Specimen
3F2/F:
(close )
Genetic Background:
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Kunming
|
Age:
|
embryonic day 3.0
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Sex:
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Not Specified
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Type:
|
optical section
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Ptk2b; red - propidium iodide (PI, for DNA).
|
Structure |
Level |
Pattern |
Image |
Note |
TS4: compacted morula |
Present |
Regionally restricted |
3F2/F
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Expression in nucleus and cytoplasm. Expression in cytoplasm not evenly distributed, but aggregated as many tiny spots.
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Specimen
3G2/G:
embryonic day 3.5
(more )
Note: Double labeled: green - Ptk2b; red - propidium iodide (PI, for DNA).
Specimen
3G2/G:
(close )
Genetic Background:
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Kunming
|
Age:
|
embryonic day 3.5
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Sex:
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Not Specified
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Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Ptk2b; red - propidium iodide (PI, for DNA).
|
Structure |
Level |
Pattern |
Image |
Note |
TS4: blastocyst |
Present |
Regionally restricted |
3G2/G
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No expression in cell nuclei. Expression distributed throughout cytoplasm. Expression in cytoplasm not evenly distributed, but aggregated as many tiny spots.
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Specimen
3H2/H:
embryonic day 4.0
(more )
Note: Double labeled: green - Ptk2b; red - propidium iodide (PI, for DNA).
Specimen
3H2/H:
(close )
Genetic Background:
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Kunming
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Age:
|
embryonic day 4.0
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Ptk2b; red - propidium iodide (PI, for DNA).
|
Structure |
Level |
Pattern |
Image |
Note |
TS5: blastocyst |
Present |
Regionally restricted |
3H2/H
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No expression in cell nuclei. Expression distributed throughout cytoplasm. Expression in cytoplasm not evenly distributed, but aggregated as many tiny spots. Expression in gaps of trophoblast cells.
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