Gene Expression Data
Assay Details
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Assay
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Reference:
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J:135407
Liao J, et al., Identification of downstream genetic pathways of Tbx1 in the second heart field. Dev Biol. 2008 Apr 15;316(2):524-37
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6385056
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Gene symbol:
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Gata4
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Gene name:
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GATA binding protein 4
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Probe:
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Gata4 probe12
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2G:
embryonic day 9.0
(more  )
Specimen
2G:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: secondary heart field |
Trace |
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2G
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Authors report low level of expression in second heart field (SHF) at E9.0-9.5
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Specimen
2H:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J * FVB * SJL
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Mutant Allele(s):
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Tbx1tm2.2Bem/Tbx1tm2.2Bem
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: secondary heart field |
Present |
Widespread |
2H
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Authors report ectopic expression thtroughout second heart field (SHF) region at E9.0.
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Specimen
E9.5 +/+ not shown:
embryonic day 9.5
(more )
Specimen
E9.5 +/+ not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: secondary heart field |
Trace |
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Authors rep.low level of expression in second heart field (SHF) at E9.0-9.5
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Specimen
E9.5 -/- not shown:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J * FVB * SJL
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Mutant Allele(s):
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Tbx1tm2.2Bem/Tbx1tm2.2Bem
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: secondary heart field |
Present |
Regionally restricted |
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Authors report ectopic expression more restricted to cranial part of second heart field (SHF) at E9.5.
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Specimen
4I:
(close )
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Genetic Background:
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involves: 129 * C3H * C57BL/6 * SJL
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Mutant Allele(s):
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Tbx1tm2.1Bem/Tbx1+
Tg(T-cre)1Lwd/0
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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Specimen
4J:
(close )
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Genetic Background:
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involves: 129 * C3H * C57BL/6 * FVB * SJL
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Mutant Allele(s):
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Tbx1tm2.1Bem/Tbx1tm2.2Bem
Tg(T-cre)1Lwd/0
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: secondary heart field |
Present |
Regionally restricted |
4J
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Authors report same changes in expression as in Tbx1-/- embryos.
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Analysis Tools