Gene Expression Data
Assay Details
Assay
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Reference:
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J:150758
Richardson GD, et al., Dynamic expression of Syndecan-1 during hair follicle morphogenesis. Gene Expr Patterns. 2009 Sep;9(6):454-60
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6386656
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Gene symbol:
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Sdc1
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Gene name:
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syndecan 1
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Antibody:
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281-2
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Detection system:
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Secondary antibody coupled to rhodamine
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Assay notes:
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Fixative was methanol/acetone.
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Results
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Specimen
2A:
embryonic day 13.5
(more )
Note: Undifferentiated dorsal skin.
Specimen
2A:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
Other - See Notes
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Embedding:
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Cryosection
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Note:
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Undifferentiated dorsal skin.
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Specimen
2B:
embryonic day 14.5
(more )
Note: Early placode stage of hair follicle morphogenesis.
Specimen
2B:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
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Note:
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Early placode stage of hair follicle morphogenesis.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: hair follicle dermal papilla |
Present |
|
2B
|
|
TS22: epidermis |
Present |
Regionally restricted |
2B
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Expression is in the outer suprabasal and differentiating layers of the epidermis.
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TS22: hair epidermal placode |
Weak |
Regionally restricted |
2B
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Expression is in the outer suprabasal and differentiating layers of the placode, is weak in the basal layer, and is absent from the newly formed placodes.
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Specimen
2C:
embryonic day 15.5
(more )
Note: Peg stage of hair follicle morphogenesis.
Specimen
2C:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
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Note:
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Peg stage of hair follicle morphogenesis.
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Specimen
2D:
embryonic day 16.5
(more )
Note: Peg stage of hair follicle morphogenesis.
Specimen
2D:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 16.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
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Embedding:
|
Cryosection
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Note:
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Peg stage of hair follicle morphogenesis.
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Specimen
2E:
embryonic day 16.5
(more )
Note: Secondary hair follicle.
Specimen
2E:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 16.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
Other - See Notes
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Embedding:
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Cryosection
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Note:
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Secondary hair follicle.
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Specimen
2F:
embryonic day 18.5
(more )
Specimen
2F:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 18.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
|
Cryosection
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Specimen
2G:
postnatal day 1
(more )
Specimen
2G:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
postnatal day 1
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
|
Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS27: hair bulb |
Present |
|
2G
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Expression is throughout all the cells of the epithelial compartment in both the primary and secondary hair follicles, with highest levels in the differentiating central column of both follicle types.
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TS27: hair follicle dermal papilla |
Weak |
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2G
|
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Specimen
2H:
postnatal day 6
(more )
Specimen
2H:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
postnatal day 6
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
Other - See Notes
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Embedding:
|
Cryosection
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Specimen
2H inset:
postnatal day 6
(more )
Specimen
2H inset:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
postnatal day 6
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
Other - See Notes
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Embedding:
|
Cryosection
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Specimen
3A:
embryonic day 13.0
(more )
Note: Early placode stage 1 of hair development.
Specimen
3A:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 13.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
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Embedding:
|
Cryosection
|
Note:
|
Early placode stage 1 of hair development.
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Specimen
3B:
embryonic day 13.0
(more )
Note: Stage 2 of hair development.
Specimen
3B:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 13.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
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Embedding:
|
Cryosection
|
Note:
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Stage 2 of hair development.
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Specimen
3C:
embryonic day 13.0
(more )
Note: Stage 3-4 of hair development.
Specimen
3C:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 13.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
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Embedding:
|
Cryosection
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Note:
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Stage 3-4 of hair development.
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Specimen
3D:
embryonic day 13.0
(more )
Note: Stage 4-5 of hair development.
Specimen
3D:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 13.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
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Embedding:
|
Cryosection
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Note:
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Stage 4-5 of hair development.
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Specimen
3E:
embryonic day 13.0
(more )
Note: Stage 5-6 of hair development.
Specimen
3E:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 13.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
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Embedding:
|
Cryosection
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Note:
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Stage 5-6 of hair development.
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Specimen
3F:
embryonic day 13.0
(more )
Note: Stage 7-8 of hair development.
Specimen
3F:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
embryonic day 13.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
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Embedding:
|
Cryosection
|
Note:
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Stage 7-8 of hair development.
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Structure |
Level |
Pattern |
Image |
Note |
TS21: vibrissa follicle |
Present |
Regionally restricted |
3F
|
Expression is highest in the inner and outer root sheath and the pre-cortex cells.
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TS21: vibrissa dermal component |
Weak |
|
3F
|
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TS21: dermis |
Present |
Regionally restricted |
3F
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Expression is at the follicle neck and in the condensed dermis surrounding the epithelial component of the hair follicle in the whisker pad.
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Specimen
3G:
embryonic day 13.0
(more )
Note: Stage 8-9 of hair development.
Specimen
3G:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 13.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
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Embedding:
|
Cryosection
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Note:
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Stage 8-9 of hair development.
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Specimen
4A:
embryonic day 18.0
(more )
Note: Tail skin stage 1 of hair development. Double labeled: red - Sdc1; green - Edar (assay MGI:6386658).
Specimen
4A:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
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Embedding:
|
Cryosection
|
Note:
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Tail skin stage 1 of hair development. Double labeled: red - Sdc1; green - Edar (assay MGI:6386658).
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Specimen
4B:
embryonic day 18.0
(more )
Note: Tail skin stage 2 of hair development. Double labeled: red - Sdc1; green - Edar (assay MGI:6386658).
Specimen
4B:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 18.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
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Tail skin stage 2 of hair development. Double labeled: red - Sdc1; green - Edar (assay MGI:6386658).
|
Specimen
4C:
embryonic day 18.0
(more )
Note: Tail skin stage 4 of hair development. Double labeled: red - Sdc1; green - Edar (assay MGI:6386658).
Specimen
4C:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Tail skin stage 4 of hair development. Double labeled: red - Sdc1; green - Edar (assay MGI:6386658).
|
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