Acta2 Immunohistochemistry Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:137692 Huang X, et al., Coronary development is regulated by ATP-dependent SWI/SNF chromatin remodeling component BAF180. Dev Biol. 2008 Jul 15;319(2):258-66
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6387271
Gene symbol:	Acta2
Gene name:	actin alpha 2, smooth muscle, aorta

Antibody:	anti-alpha-SMA
Detection system:	Alexa Fluor 594

Results

S2_-/- (upper) ©

Specimen S2 -/- (upper): embryonic day 14.5; Pbrm1^tm1Rtj/Pbrm1^tm1Rtj (more

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Note: Triple labeled: red - Acta2; green - Pecam1 (assay MGI:6387272); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: blood vessel smooth muscle	Present		S2_-/- (upper)

S2_-/- (middle) ©

Specimen S2 -/- (middle): embryonic day 14.5; Pbrm1^tm1Rtj/Pbrm1^tm1Rtj (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (assay MGI:6387272); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: blood vessel smooth muscle	Present		S2_-/- (middle)

S2_-/- (lower) ©

Specimen S2 -/- (lower): embryonic day 14.5; Pbrm1^tm1Rtj/Pbrm1^tm1Rtj (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (assay MGI:6387272); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: blood vessel smooth muscle	Present		S2_-/- (lower)

S2_+/+ (upper) ©

Specimen S2 +/+ (upper): embryonic day 14.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (assay MGI:6387272); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: blood vessel smooth muscle	Present		S2_+/+ (upper)

S2_+/+ (middle) ©

Specimen S2 +/+ (middle): embryonic day 14.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (assay MGI:6387272); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: blood vessel smooth muscle	Present		S2_+/+ (middle)

S2_+/+ (lower) ©

Specimen S2 +/+ (lower): embryonic day 14.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (assay MGI:6387272); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: blood vessel smooth muscle	Present		S2_+/+ (lower)

6B -/- ©

Specimen 6B -/-: embryonic day 13.5; Pbrm1^tm1Rtj/Pbrm1^tm1Rtj (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (assay MGI:6387272); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS21: ascending aorta	Present	Regionally restricted	6B -/-	No difference in expression between wild type and mutant cells surrounding aorta and pulmonary trunk arteries.
TS21: pulmonary trunk	Present	Regionally restricted	6B -/-	No difference in expression between wild type and mutant cells surrounding aorta and pulmonary trunk arteries.

6B +/+ ©

Specimen 6B +/+: embryonic day 13.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (assay MGI:6387272); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS21: ascending aorta	Present	Regionally restricted	6B +/+	No difference in expression between wild type and mutant cells surrounding aorta and pulmonary trunk arteries.
TS21: pulmonary trunk	Present	Regionally restricted	6B +/+	No difference in expression between wild type and mutant cells surrounding aorta and pulmonary trunk arteries.

6C -/- (upper) ©

Specimen 6C -/- (upper): embryonic day 13.5; Pbrm1^tm1Rtj/Pbrm1^tm1Rtj (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (assay MGI:6387272); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS21: blood vessel smooth muscle	Present	Regionally restricted	6C -/- (upper)	Expression in a significant number of individual cells within subepicardial space.

6C -/- (lower) ©

Specimen 6C -/- (lower): embryonic day 13.5; Pbrm1^tm1Rtj/Pbrm1^tm1Rtj (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (assay MGI:6387272); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS21: blood vessel smooth muscle	Present	Regionally restricted	6C -/- (lower)	Expression in disorganized smooth muscle cells inside nodules.

6C +/- (upper) ©

Specimen 6C +/- (upper): embryonic day 13.5; Pbrm1^tm1Rtj/Pbrm1⁺ (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (assay MGI:6387272); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS21: blood vessel smooth muscle	Present		6C +/- (upper)

6C +/- (lower) ©

Specimen 6C +/- (lower): embryonic day 13.5; Pbrm1^tm1Rtj/Pbrm1⁺ (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (assay MGI:6387272); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS21: blood vessel smooth muscle	Present		6C +/- (lower)

6D -/- ©

Specimen 6D -/-: embryonic day 14.5; Pbrm1^tm1Rtj/Pbrm1^tm1Rtj (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (assay MGI:6387272); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: blood vessel smooth muscle	Present	Regionally restricted	6D -/-	Expression in large number of smooth muscle cells inside larger nodules.

6D +/- ©

Specimen 6D +/-: embryonic day 14.5; Pbrm1^tm1Rtj/Pbrm1⁺ (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (assay MGI:6387272); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: blood vessel smooth muscle	Present		6D +/-

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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