Gene Expression Data
Assay Details
Assay
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Reference:
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J:196346
Burgold T, et al., The H3K27 demethylase JMJD3 is required for maintenance of the embryonic respiratory neuronal network, neonatal breathing, and survival. Cell Rep. 2012 Nov 29;2(5):1244-58
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6403162
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Gene symbol:
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Kdm6b
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Gene name:
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KDM1 lysine (K)-specific demethylase 6B
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Probe:
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Jmjd3 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4E:
embryonic day 18.5
(more )
Note: Double labeled: blue - Kdm6b; red - Tacr1 (assay MGI:6403157).
Specimen
4E:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Kdm6b; red - Tacr1 (assay MGI:6403157).
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Structure |
Level |
Pattern |
Image |
Note |
TS26: brainstem nucleus |
Present |
Regionally restricted |
4E
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Expression in the brainstem was predominant in the pre-Botzinger complex, the nucleus Ambiguus, and the facial motor nucleus. Expression was much lower if barely detectable in the retrotrapezoid nucleus/parafacial respiratory group.
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Specimen
S3D Jmjd3+/+:
embryonic day 18.5
(more )
Specimen
S3D Jmjd3+/+:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S3D Jmjd3-/-:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Kdm6bGt(XB814)Byg/Kdm6bGt(XB814)Byg
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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