Gene Expression Data
Assay Details
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Assay
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Reference:
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J:152494
Behesti H, et al., Loss of Tbx2 delays optic vesicle invagination leading to small optic cups. Dev Biol. 2009 Sep 15;333(2):360-72
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6404556
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Gene symbol:
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Nefm
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Gene name:
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neurofilament, medium polypeptide
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Antibody:
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RMO-270
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Detection system:
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fluorescein
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Results
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Specimen
7A:
embryonic day 12.5
(more  )
Note: Double labeled: green - Nefm; red - propidium iodide.
Specimen
7A:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6 * ICR
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Nefm; red - propidium iodide.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: neural retina |
Present |
Regionally restricted |
7A
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Expression in retinal ganglion cell (RGC) bodies and processes. Expression in organized nerve fiber layer dorsal to optic disc.
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Specimen
7B:
embryonic day 12.5; Tbx2tm1Pa/ Tbx2tm1Pa
(more )
Note: Double labeled: green - Nefm; red - propidium iodide.
Specimen
7B:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * ICR
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Mutant Allele(s):
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Tbx2tm1Pa/Tbx2tm1Pa
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Nefm; red - propidium iodide.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: neural retina |
Present |
Regionally restricted |
7B
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Expression in organized nerve fiber layer dorsal to optic disc.
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Specimen
7C:
embryonic day 12.5
(more )
Note: Double labeled: green - Nefm; red - propidium iodide.
Specimen
7C:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * ICR
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Nefm; red - propidium iodide.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: neural retina |
Present |
Regionally restricted |
7C
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Retinal ganglion cell (RGC) axons follow vitreal surface towards optic nerve head.
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Specimen
7D:
embryonic day 12.5; Tbx2tm1Pa/ Tbx2tm1Pa
(more )
Note: Double labeled: green - Nefm; red - propidium iodide.
Specimen
7D:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * ICR
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Mutant Allele(s):
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Tbx2tm1Pa/Tbx2tm1Pa
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Nefm; red - propidium iodide.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: neural retina |
Present |
Regionally restricted |
7D
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Retinal ganglion cell (RGC) axons were disorganized; no axons exiting the optic disc.
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Specimen
7E:
embryonic day 12.5
(more )
Note: Double labeled: green - Nefm; red - propidium iodide.
Specimen
7E:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * ICR
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
|
Double labeled: green - Nefm; red - propidium iodide.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: neural retina |
Present |
Regionally restricted |
7E
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Retinal ganglion cell (RGC) axons follow vitreal surface towards optic nerve head.
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Specimen
7F:
embryonic day 12.5; Tbx2tm1Pa/ Tbx2tm1Pa
(more )
Note: Double labeled: green - Nefm; red - propidium iodide.
Specimen
7F:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * ICR
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Mutant Allele(s):
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Tbx2tm1Pa/Tbx2tm1Pa
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
|
Double labeled: green - Nefm; red - propidium iodide.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: neural retina |
Present |
Regionally restricted |
7F
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Retinal ganglion cell (RGC) axons were disorganized; no axons exiting the optic disc.
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Analysis Tools