Antibody:	anti-Nephronectin
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: embryo ectoderm	Present		7A
TS17: gut	Present		7A
TS17: nephric duct	Present		7A

Structure	Level	Pattern	Image	Note
TS17: nephric duct	Present		7B
TS17: trunk mesenchyme	Present	Regionally restricted	7B	Expression is at interfaces between differentiating somites.

Structure	Level	Pattern	Image	Note
TS21: metanephros	Strong	Regionally restricted	7C	Expression is at the interface between epithelial cells of the ureter and the surrounding metanephric mesenchyme and between epithelial cells of the ureteric bud and the surrounding mesenchyme.
TS21: primitive collecting duct	Strong		7C

Structure	Level	Pattern	Image	Note
TS19: nephric duct	Present		7C inset
TS19: ureteric bud	Present		7C inset

Structure	Level	Pattern	Image	Note
TS21: metanephros	Strong	Regionally restricted	7E	Expression is in the basal lamina between the ureteric bud and metanephric mesenchyme.
TS21: comma-shaped body	Trace		7E	There is little or no expression.

Structure	Level	Pattern	Image	Note
TS21: primitive collecting duct	Present		7G

Structure	Level	Pattern	Image	Note
TS21: primitive collecting duct	Strong	Regionally restricted	7I	Expression is in the portion of the tubule derived from the ureteric bud.
TS21: s-shaped body	Weak		7I

Structure	Level	Pattern	Image	Note
TS26: glomerular tuft	Strong		7J
TS26: collecting duct	Present		7J

Structure	Level	Pattern	Image	Note
TS27: lens	Present	Uniform	9A

Structure	Level	Pattern	Image	Note
TS22: inner ear	Present	Regionally restricted	9B	Expression is in basal lamina surrounding epithelium and on apical surface of epithelia.
TS22: inner ear epithelium	Present	Regionally restricted	9B	Expression is in basal lamina surrounding epithelium and on apical surface of epithelia.

Structure	Level	Pattern	Image	Note
TS21: choroid plexus	Strong	Regionally restricted	9C	Expression is in basal lamina and is strongly expressed by epithelial cells.

Structure	Level	Pattern	Image	Note
TS27: taste bud	Present		9D
TS27: tongue muscle	Weak		9D

Structure	Level	Pattern	Image	Note
TS21: remnant of Rathke's pouch	Present	Regionally restricted	9E	Expression is in the basal lamina surrounding the organ.

Structure	Level	Pattern	Image	Note
TS21: oral epithelium	Present	Regionally restricted	9F	Expression is in the basal lamina underlying the oral epithelium.
TS21: oral region	Present	Regionally restricted	9F	Expression is in the basal lamina underlying the oral epithelium.
TS21: epidermis	Present	Regionally restricted	9F	Expression is in the basal lamina underlying the skin.
TS21: skin	Present	Regionally restricted	9F	Expression is in the basal lamina underlying the skin.
TS21: jaw mesenchyme	Present	Regionally restricted	9F	Expression is surrounding a developing incisor.

Structure	Level	Pattern	Image	Note
TS25: lung epithelium	Present	Regionally restricted	9G	Expression is in the basal lamina surrounding the branching epithelia.
TS25: lung mesenchyme	Present		9G

Structure	Level	Pattern	Image	Note
TS21: esophagus	Present		9H	Expression is in the basal lamina.
TS21: stomach	Present		9H	Expression is in the basal lamina.
TS21: pancreas	Weak		9H
TS21: liver	Trace		9H