About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:221356 Snowball J, et al., Mesenchymal Wnt signaling promotes formation of sternum and thoracic body wall. Dev Biol. 2015 May 15;401(2):264-75
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6407008
Gene symbol: Acta2
Gene name: actin alpha 2, smooth muscle, aorta
Results
Specimen 2G: embryonic day 12.5; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Triple labeled: red - Acta2; blue - Sox9 (assay MGI:6406997); green - Cre reporter.
Structure Level Pattern Image Note
TS20: body wall Present Regionally restricted 2G Expression in ventral body wall.
Specimen 2H: embryonic day 12.5; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Triple labeled: red - Acta2; blue - Sox9 (assay MGI:6406997); green - Cre reporter.
Structure Level Pattern Image Note
TS20: body wall Present Regionally restricted 2H , 2H' Expression in ventral body wall.
Specimen 4B Control: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: pectoral girdle and thoracic body wall musculature Present 4B Control Authors report expression in skeletal and smooth muscle was similar to that of controls.
Specimen 4B Wls f/f; Dermo1 Cre/+: embryonic day 14.5; Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: pectoral girdle and thoracic body wall musculature Present 4B Wls f/f; Dermo1 Cre/+ Authors report expression in skeletal and smooth muscle was similar to that of controls.
Specimen 4C Control: embryonic day 14.5 (more )
Note: Double labeled: red - Acta2; green - Sox9 (assay MGI:6406997).
Structure Level Pattern Image Note
TS22: thoracic body wall Present Regionally restricted 4C Control Expression between sternal bars.
Specimen 4C Wls f/f; Dermo1 Cre/+: embryonic day 14.5; Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; green - Sox9 (assay MGI:6406997).
Structure Level Pattern Image Note
TS22: pectoral girdle and thoracic body wall musculature Present 4C Wls f/f; Dermo1 Cre/+
Specimen 7C Control (upper): embryonic day 14.5 (more )
Note: Double labeled: red - Acta2; green - Sox9 (assay MGI:6406997).
Structure Level Pattern Image Note
TS22: pectoral girdle and thoracic body wall musculature Present Regionally restricted 7C Control (upper) Expression in myocytes appearing as leading cells between almost fused sternal bars.
Specimen 7C Wls f/f; Dermo1 Cre/+: embryonic day 14.5; Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; green - Sox9 (assay MGI:6406997).
Structure Level Pattern Image Note
TS22: thoracic body wall Present Regionally restricted 7C Wls f/f; Dermo1 Cre/+ Expression in poorly organized cells in ventral body wall.
Specimen 7C Control (lower): embryonic day 14.5 (more )
Note: Double labeled: red - Acta2; green - Sox9 (assay MGI:6406997).
Structure Level Pattern Image Note
TS22: pectoral girdle and thoracic body wall musculature Present Regionally restricted 7C Control (lower) Expression in myocytes appearing as leading cells between almost fused sternal bars.
Specimen 7C LRP5/6 f/f; Dermo1 Cre/+: embryonic day 14.5; Lrp5tm1.1Vari/Lrp5tm1.1Vari, Lrp6tm1.1Vari/Lrp6tm1.1Vari, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Double labeled: red - Acta2; green - Sox9 (assay MGI:6406997).
Structure Level Pattern Image Note
TS22: thoracic body wall Present Regionally restricted 7C LRP5/6 f/f; Dermo1 Cre/+ Expression in poorly organized cells in ventral body wall.
Specimen S3B Control (1st row): embryonic day 13.5 (more )
Note: Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS21: pectoral girdle and thoracic body wall musculature Present S3B Control (1st row)
Specimen S3B Wls f/f; Dermo1 Cre/+ (1st row): embryonic day 13.5; Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS21: pectoral girdle and thoracic body wall musculature Present S3B Wls f/f; Dermo1 Cre/+ (1st row) Authors report expression in skeletal and smooth muscle was similar to that of controls.
Specimen S3B Control BW (2nd row): embryonic day 13.5 (more )
Note: Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS21: pectoral girdle and thoracic body wall musculature Present S3B Control (2nd row)
Specimen S3B Wls f/f; Dermo1 Cre/+ BW (2nd row): embryonic day 13.5; Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS21: pectoral girdle and thoracic body wall musculature Present S3B Wls f/f; Dermo1 Cre/+ (2nd row) Authors report expression in skeletal and smooth muscle was similar to that of controls.
Specimen Double labeled: red - Acta2; blue - DAPI.S3B Control BW (3rd row): embryonic day 13.5 (more )
Note: Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS21: pectoral girdle and thoracic body wall musculature Present S3B Control (3rd row)
Specimen S3B Wls f/f; Dermo1 Cre/+ BW (3rd row): embryonic day 13.5; Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS21: pectoral girdle and thoracic body wall musculature Present S3B Wls f/f; Dermo1 Cre/+ (3rd row) Authors report expression in skeletal and smooth muscle was similar to that of controls.
Specimen S3B Control Fl (4th row): embryonic day 13.5 (more )
Note: Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS21: pectoral girdle and thoracic body wall musculature Present S3B Control (4th row)
Specimen S3B Wls f/f; Dermo1 Cre/+ Fl (4th row): embryonic day 13.5; Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS21: pectoral girdle and thoracic body wall musculature Present S3B Wls f/f; Dermo1 Cre/+ (4th row) Authors report expression in skeletal and smooth muscle was similar to that of controls.
Specimen S4 Control (1st row left): embryonic day 11.5 (more )
Note: Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_Control (top row left)
Specimen S4 Wls f/f;Dermo1 Cre/+ (1st row): embryonic day 11.5; Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_Wls f/f; Dermo1 Cre/+ (top row)
Specimen S4 Control (2nd row left): embryonic day 11.5 (more )
Note: Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_Control Body wall (left)
Specimen S4 Wls f/f;Dermo1 Cre/+ (2nd row): embryonic day 11.5; Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_Wls f/f; Dermo1 Cre/+ Body wall
Specimen S4 Control (3rd row left): embryonic day 11.5 (more )
Note: Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_Control Flanks (left)
Specimen S4 Wls f/f;Dermo1 Cre/+ (3rd row): embryonic day 11.5; Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_Wls f/f; Dermo1 Cre/+ Flanks
Specimen S4 Control (1st row right): embryonic day 11.5 (more )
Note: Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_Control (top row right)
Specimen S4 LRP5/6 f/f Dermo1 Cre/+ (1st row): embryonic day 11.5; Lrp5tm1.1Vari/Lrp5tm1.1Vari, Lrp6tm1.1Vari/Lrp6tm1.1Vari, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_LRP5/6 f/f; Dermo1 Cre/+ (top row)
Specimen S4 Control (2nd row right): embryonic day 11.5 (more )
Note: Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_Control Body wall (right)
Specimen S4 LRP5/6 f/f Dermo1 Cre/+ (2nd row): embryonic day 11.5; Lrp5tm1.1Vari/Lrp5tm1.1Vari, Lrp6tm1.1Vari/Lrp6tm1.1Vari, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_LRP5/6 f/f; Dermo1 Cre/+ Body wall
Specimen S4 Control (3rd row right): embryonic day 11.5 (more )
Note: Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_Control Flanks (right)
Specimen S4 LRP5/6 f/f Dermo1 Cre/+ (3rd row): embryonic day 11.5; Lrp5tm1.1Vari/Lrp5tm1.1Vari, Lrp6tm1.1Vari/Lrp6tm1.1Vari, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_LRP5/6 f/f; Dermo1 Cre/+ Flanks
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory