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Gene Expression Data
Assay Details
Assay
Reference: J:166736 Tee WW, et al., Prmt5 is essential for early mouse development and acts in the cytoplasm to maintain ES cell pluripotency. Genes Dev. 2010 Dec 15;24(24):2772-7
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6415680
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-OCT4 (clone 40; #611202)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1A 8-cell: embryonic day 2.5 (more )
Note: 8-cell stage embryo. Double labeled: green - Pou5f1; red - Prmt5 (assay MGI:6415679).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 1A Oct4 8-cell , 1A 8-cell Merge
Specimen 1A Morula: embryonic day 3.0 (more )
Note: Morula stage embryo. Double labeled: green - Pou5f1; red - Prmt5 (assay MGI:6415679).
Structure Level Pattern Image Note
TS3: compacted morula Present 1A Oct4 Morula , 1A Morula Merge
Specimen 1A E3.5: embryonic day 3.5 (more )
Note: Early blastocyst stage embryo. Double labeled: green - Pou5f1; red - Prmt5 (assay MGI:6415679).
Structure Level Pattern Image Note
TS4: inner cell mass Present 1A E3.5 Merge , 1A Oct4 E3.5
Specimen 1A E4.5: embryonic day 4.5 (more )
Note: Double labeled: green - Pou5f1; red - Prmt5 (assay MGI:6415679).
Structure Level Pattern Image Note
TS6: epiblast Present 1A E4.5 Merge , 1A Oct4 E4.5
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
MGI 6.13 		The Jackson Laboratory