Probe:	Robo3 probe2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: rhombic lip	Strong	Regionally restricted	1q	High expression in rhombic lip neurons in hindbrain.
TS20: hindbrain	Present	Regionally restricted	1q	Expression in commissural axons crossing midline all along midbrain, hindbrain and spinal cord.
TS20: midbrain	Present	Regionally restricted	1q	Expression in commissural axons crossing midline all along midbrain, hindbrain and spinal cord.
TS20: spinal cord	Present	Regionally restricted	1q	Expression in commissural axons crossing midline all along midbrain, hindbrain and spinal cord.

Structure	Level	Pattern	Image	Note
TS22: cerebellum ventricular layer	Present		2c
TS22: deep cerebellar nucleus	Present		2c

Structure	Level	Pattern	Image	Note
TS22: brainstem nucleus	Present	Regionally restricted	2d	Expression in migrating lateral reticular nuclei neurons.

Structure	Level	Pattern	Image	Note
TS24: nerve fiber tract	Present	Regionally restricted	2f	Expression in migrating pontine neurons in anterior extramural migratory stream (Aems). Expression in pontine neurons stops before neurons reach ventral midline.

Structure	Level	Pattern	Image	Note
TS26: nerve fiber tract	Trace	Regionally restricted	2g	Faint expression in migrating pontine neurons. Anterior extramural migratory stream (Aems) reduced to thin stream of expressing cells.

Structure	Level	Pattern	Image	Note
TS22: medial habenular nucleus	Present	Regionally restricted	2h	Expression in medial habenula neurons.

Structure	Level	Pattern	Image	Note
TS16: future spinal cord	Present	Regionally restricted	4a	Expression in brachial spinal cord, first superficially in a few neurons in dorsal part of spinal cord. Expression in commissural neurons in marginal zone.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	4b	Expression in brachial spinal cord, only detected in two symmertrical dorsal-ventral bands of newly postitoic neurons adjacent to ventricular zone. Expression in small ventral domains probably corresponding to V3 interneurons. Expression rapidly dowregulated after midline crossing.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	4c	Expression in brachial spinal cord, dorsally adjacent to ventricular zone, but also ventrally at level of V3 interneurons. Expression down-regulated at later developmental stages. No expression in older more superficial neurons.

Structure	Level	Pattern	Image	Note
TS22: spinal cord ventricular layer	Weak		4d	Expression in brachial spinal cord, expression in few neurons along ventricular zone.
TS22: spinal cord	Weak	Regionally restricted	4d	Expression down-regulated at later developmental stages. No expression in older more superficial neurons.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	6e	Expression in medial and lateral ganglion eminences. Expression in some cortical plate neurons