Gene Expression Data
Assay Details
Assay
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Reference:
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J:278498
Friocourt F, et al., Shared and differential features of Robo3 expression pattern in amniotes. J Comp Neurol. 2019 Aug 15;527(12):2009-2029
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6416077
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Gene symbol:
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Robo3
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Gene name:
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roundabout guidance receptor 3
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Probe:
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Robo3 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1q:
embryonic day 12.5
(more )
Specimen
1q:
(close )
Genetic Background:
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Rj:SWISS
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS20: rhombic lip |
Strong |
Regionally restricted |
1q
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High expression in rhombic lip neurons in hindbrain.
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TS20: hindbrain |
Present |
Regionally restricted |
1q
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Expression in commissural axons crossing midline all along midbrain, hindbrain and spinal cord.
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TS20: midbrain |
Present |
Regionally restricted |
1q
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Expression in commissural axons crossing midline all along midbrain, hindbrain and spinal cord.
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TS20: spinal cord |
Present |
Regionally restricted |
1q
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Expression in commissural axons crossing midline all along midbrain, hindbrain and spinal cord.
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Specimen
2c:
embryonic day 14.5
(more )
Specimen
2c:
(close )
Genetic Background:
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Rj:SWISS
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2d:
embryonic day 14.5
(more )
Specimen
2d:
(close )
Genetic Background:
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Rj:SWISS
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS22: brainstem nucleus |
Present |
Regionally restricted |
2d
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Expression in migrating lateral reticular nuclei neurons.
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Specimen
2f:
embryonic day 16.5
(more )
Specimen
2f:
(close )
Genetic Background:
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Rj:SWISS
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS24: nerve fiber tract |
Present |
Regionally restricted |
2f
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Expression in migrating pontine neurons in anterior extramural migratory stream (Aems). Expression in pontine neurons stops before neurons reach ventral midline.
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Specimen
2g:
embryonic day 18.5
(more )
Specimen
2g:
(close )
Genetic Background:
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Rj:SWISS
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS26: nerve fiber tract |
Trace |
Regionally restricted |
2g
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Faint expression in migrating pontine neurons. Anterior extramural migratory stream (Aems) reduced to thin stream of expressing cells.
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Specimen
2h:
embryonic day 14.5
(more )
Specimen
2h:
(close )
Genetic Background:
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Rj:SWISS
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4a:
embryonic day 10.0
(more )
Specimen
4a:
(close )
Genetic Background:
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Rj:SWISS
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Age:
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embryonic day 10.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS16: future spinal cord |
Present |
Regionally restricted |
4a
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Expression in brachial spinal cord, first superficially in a few neurons in dorsal part of spinal cord. Expression in commissural neurons in marginal zone.
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Specimen
4b:
embryonic day 11.5
(more )
Specimen
4b:
(close )
Genetic Background:
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Rj:SWISS
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Present |
Regionally restricted |
4b
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Expression in brachial spinal cord, only detected in two symmertrical dorsal-ventral bands of newly postitoic neurons adjacent to ventricular zone. Expression in small ventral domains probably corresponding to V3 interneurons. Expression rapidly dowregulated after midline crossing.
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Specimen
4c:
embryonic day 13.0
(more )
Specimen
4c:
(close )
Genetic Background:
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Rj:SWISS
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Age:
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embryonic day 13.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Regionally restricted |
4c
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Expression in brachial spinal cord, dorsally adjacent to ventricular zone, but also ventrally at level of V3 interneurons. Expression down-regulated at later developmental stages. No expression in older more superficial neurons.
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Specimen
4d:
embryonic day 14.5
(more )
Specimen
4d:
(close )
Genetic Background:
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Rj:SWISS
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS22: spinal cord ventricular layer |
Weak |
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4d
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Expression in brachial spinal cord, expression in few neurons along ventricular zone.
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TS22: spinal cord |
Weak |
Regionally restricted |
4d
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Expression down-regulated at later developmental stages. No expression in older more superficial neurons.
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Specimen
6e:
embryonic day 11.5
(more )
Specimen
6e:
(close )
Genetic Background:
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Rj:SWISS
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS19: telencephalon |
Present |
Regionally restricted |
6e
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Expression in medial and lateral ganglion eminences. Expression in some cortical plate neurons
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