Erg Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:156019 Nikolova-Krstevski V, et al., ERG is required for the differentiation of embryonic stem cells along the endothelial lineage. BMC Dev Biol. 2009;9:72
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6431000
Gene symbol:	Erg
Gene name:	ETS transcription factor

Antibody:	anti-ERG
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

3A Erg ©

3A left ©

3A Merge ©

Specimen 3A: embryonic day 9.5 (more

)
Note: Double labeled: green - Erg; red - Cdh5 (assay MGI:6431002).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present	Regionally restricted	3A left , 3A Merge , 3A Erg	Expression is in the endothelium.

3B Erg ©

3B Merge ©

Specimen 3B: embryonic day 12.5 (more

)
Note: Double labeled: green - Erg; red - Cdh5 (assay MGI:6431002).

Structure	Level	Pattern	Image	Note
TS20: dorsal aorta	Present	Regionally restricted	3B Merge , 3B Erg	Expression is in the vessel wall with faint staining in a few cells in the lumen.
TS20: blood vessel endothelium	Present		3B Merge , 3B Erg

4_bottom Erg ©

4_top Erg ©

4_top Merge ©

Specimen 4: embryonic day 9.5 (more

)
Note: Double labeled: green - Erg; red - Cdh5 (assay MGI:6431002).

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle endocardial lining	Present		4_top Merge , 4_top Erg
TS15: common atrial chamber endocardial lining	Present		4_bottom Erg , 4_bottom Merge

S5a ©

S5b ©

S5c ©

S5d ©

S5e ©

S5f ©

Specimen S5: embryonic day 12.5 (more

)
Note: Double labeled: green - Erg; red - Cdh5 (assay MGI:6431002).

Structure	Level	Pattern	Image	Note
TS20: blood vessel endothelium	Present		S5e , S5b , S5c , S5f , S5a , S5d
TS20: intersomitic blood vessel	Present		S5e , S5b , S5c , S5f , S5a , S5d

S6Ba ©

S6Bc ©

S6Bd ©

Specimen S6: embryonic day 12.5 (more

)
Note: Double labeled: green - Erg; red - Cdh5 (assay MGI:6431002).

Structure	Level	Pattern	Image	Note
TS20: blood vessel endothelium	Present		S6Bd , S6Bc , S6Ba	Expression is in the developing vasculature in the aorta-gonado-mesonephros region.

S7Ba ©

S7Bc ©

S7Bd ©

S7Ce ©

S7Cg ©

S7Ch ©

Specimen S7: embryonic day 12.5 (more

)
Note: Double labeled: green - Erg; red - Cdh5 (assay MGI:6431002).

Structure	Level	Image
TS20: umbilical artery	Present	S7Ba , S7Bd , S7Bc
TS20: umbilical vein	Present	S7Ce , S7Cg , S7Ch
TS20: blood vessel endothelium	Present	S7Ch , S7Bc , S7Ce , S7Bd , S7Ba , S7Cg

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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